Human IL-1 beta/IL-1F2 Antibody (2805)

R&D Systems | Catalog # MAB601

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Dengue virus

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, Affinity Chromatography, Antibody Array Development, Array Development, Bioassay, Electrochemiluminescent Assay, ELISA (Capture), ELISA Capture, ELISA Development, ELISA Development (Capture), Luminex Development, Neutralizing

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2805
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-1 beta /IL-1F2

Specificity

Detects human IL-1 beta /IL-1F2 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 4% cross-reactivity with recombinant rat (rr) IL‑1 beta and less than 0.1% with recombinant porcine (rp) IL-1 beta, recombinant human IL-1 alpha, rpIL-1 alpha, rrIL-1 alpha, recombinant mouse (rm) IL-1 alpha, and rmIL-1 beta is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-1 beta/IL-1F2 Antibody (2805)

Detection of Human IL-1 beta /IL-1F2 antibody by Western Blot.

Detection of Human IL‑1 beta /IL‑1F2 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta /IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IL‑1 beta /IL‑1F2 in THP‑1 Human Cell Line.

IL‑1 beta /IL‑1F2 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line using Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of THP-1 cells treated with 200nM PMA for 24 hours then 10ug/mL LPS for 24 hours. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IL-1 beta /IL-1F2 antibody in Human PBMCs by Immunocytochemistry (ICC).

IL‑1 beta /IL‑1F2 in Human PBMCs.

IL-1 beta /IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human IL‑1 beta /IL‑1F2 by Simple WesternTM.

Simple Western lane view shows lysates of THP‑1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nm PMA and 10 ug/ml LPS for 24 hrs and 3 hrs, respectively, and loaded at 0.2 mg/mL. A specific band was detected for IL‑1 beta /IL‑1F2 at approximately 38 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody.

Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Human IL‑1 beta /IL‑1F2 Antibody.

Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (0.05 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB601). The ND50 is typically 0.05-0.2 µg/mL.
Detection of Human IL-1 beta/IL-1F2 by ELISA

Detection of Human IL-1 beta/IL-1F2 by ELISA

Effect of A1AT on whole blood IL-1 beta release. IL-1 beta production in whole blood cultures in response to LPS (1.0 μg/ml) was performed in the presence of endogenous A1AT (i.e., undiluted) or exogenously added A1AT (2 mg/ml) in blood diluted 1:32 with RPMI. Whole blood cultures were incubated for 18 h. After incubation, plasma supernatants were removed, and IL-1 beta quantified by ELISA and expressed as mean ± SD for three donors. The diluted sample result was corrected for the dilution. NS indicates no significant difference. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0117330), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry

Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Western Blot

Detection of Human IL-1 beta/IL-1F2 by Western Blot

Effect of high glucose and IL-1 beta alone or in combination on the protein expressions of CD31, FSP1 and alpha -SMA in HAECs. a–d HAECs were incubated for 48 h with NG and HG. Mannitol was used as a control for hyperosmolarity. Representative western blots (a) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (b–d) are presented. e–h HAECs were treated for 48 h with NG, HG, IL-1 beta (10 ng/ml)and HG in the presence of the IL-1 beta (10 ng/ml). Representative western blots (E) and quantitative determinations of CD31, FSP1 and alpha -SMA protein levels (f–h) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), MN 5.5 mM glucose + 24.5 mM mannitol, IL-1 beta (10 ng/ml), HG + IL-1 beta : high glucose (30 mM) + IL-1 beta (10 ng/ml) *P < 0.05 vs. MN or NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Western Blot

Detection of Human IL-1 beta/IL-1F2 by Western Blot

Effects of PKC beta on high glucose induced IL-1 beta up-regulation. Confluent cultures of HAECs were exposed to NG, HG, PMA (30 nM) and HG in the presence of the selective PKC beta inhibitors (LY317615, 0.3 μM) for 48 h. Real-time PCR analyses showed mRNA expression of PKC beta and IL-1 beta (a, b). Representative western blots (c) and quantitative determinations of PKC beta and IL-1 beta (d, e) are presented. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM), PMA (30 nM): phorbol 12-myristate13-acetate; LY (0.3 uM): LY317615; *P < 0.05 vs.NG, **P < 0.01 vs. NG, #P < 0.05 vs. HG or PMA Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry

Detection of Mouse IL-1 beta/IL-1F2 by Immunohistochemistry

Inflammatory response induced by HI injury and maternal SE. Representative images of immunofluorescence staining of inflammatory cytokines IL-1 beta, IL-6, and TNF alpha in the cerebral cortex (A) and the hippocampus (E). The immunofluorescence intensity of IL-1 beta (B,F), IL-6 (C,G), and TNF alpha (D,H) in the cerebral cortex and hippocampus. Results are presented as mean ± SEM. *P < 0.05, **P < 0.01, n = 4, analyzed by two-way ANOVA followed by post hoc Turkey tests. SH, from sham exposed dams with sham surgery; HI, hypoxic-ischemic injury; SE, from smoke exposed dams with sham surgery; HI + SE, from smoke exposed dams with hypoxic-ischemic injury. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35250486), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

Detection of Human IL-1 beta/IL-1F2 by Immunocytochemistry/Immunofluorescence

The influence of high glucose or IL-1 beta on immunofluorescence of CD31 and FSP1 in HAECs. Representative immunofluorescence images showing CD31 (green), FSP1 (red) labeling and DAPI (blue) stains nuclei. a Normal ECs monolayers displayed a cobble stone morphology. b A merge of the three images revealed some cells populations that acquired a spindle-shaped morphology and lost CD31 expression (white arrow). c HAECs exposure to IL-1 beta alone for 48 h acquired a spindle-shaped morphology. d High glucose and IL-1 beta in combination resulted in decreased CD31 (the left white arrow) and increased FSP1staining (the right arrow). a normal glucose (5.5 mM) group, b high glucose (30 mM) group for 48 h; c treatment with a normal glucose (5.5 mM) + IL-1 beta (10 ng/ml) treatment for 48 h, d treatment with a high glucose (30 mM) + IL-1 beta (10 ng/ml) treatment for 48 h. Scale bar, 75 μm Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta/IL-1F2 by Western Blot

Detection of Human IL-1 beta/IL-1F2 by Western Blot

The influence of blocking IL-1 beta treatment on the protein expressions of CD31, FSP1, a-SMA, and IL-1 beta. (a–f) HAECs were incubated for 48 h with anti-IL-1 beta antibodies (1000 ng/ml) in the presence of NG or HG. (a1–f1) We performed gene-silencing experiments using transfection with siRNA specific for IL-1 beta. The protein expressions of IL-1 beta, CD31, FSP1 and alpha -SMA were assessed by western blotting. The data are expressed as the mean ± SD. Experiments were repeated at least three times. NG normal glucose (5.5 mM), HG high glucose (30 mM). Anti-IL-1 beta : anti-IL-1 beta antibodies (1000 ng/ml). *P < 0.05 vs. NG or anti-IL-1 beta, #P < 0.05 vs. HG or HG +Vehicle Image collected and cropped by CiteAb from the following publication (https://www.cardiab.com/content/15/1/42), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human IL-1 beta /IL-1F2 by Western Blot

Detection of Human IL-1 beta /IL-1F2 by Western Blot

Knockdown of IRE1 alpha reduces LPS-induced cytokine production.THP-1 cells transfected with either noncoding (NC) or IRE1 alpha targeting siRNA were treated with either 1 μg/ml LPS alone for 24 h or 1 μg/ml LPS for 24 h followed by addition of 10 μM nigericin (NG) for 45 min. a Cell lysates were analysed via immunoblotting for IRE1 alpha, phospho-p65, total p65, NLRP3, pro-caspase-1 and pro-IL-1 beta. Actin was used as a loading control. b Cell conditioned medium was analysed via immunoblotting for pro-IL-1 beta processing. c–f Levels of IL-1 beta, IL-8, TNF-alpha, and IL-6 were assayed in cell conditioned medium by ELISA (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 based on a Student’s t test. Error bars represent SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31417078), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Oral live L. plantarum GKD7 reduced levels of TNF-alpha and IL-1 beta expression in OA synovium. (A) IHC staining of IL-1 beta and TNF-alpha in representative synovium from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in synovium. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Oral live L. plantarum GKD7 reduced levels of TNF-alpha and IL-1 beta expression in OA synovium. (A) IHC staining of IL-1 beta and TNF-alpha in representative synovium from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in synovium. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Oral live L. plantarum GKD7 reduced key proinflammatory cytokines in OA cartilage. (A) IHC staining of IL-1 beta and TNF-alpha expression in representative cartilage from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in cartilage. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Detection of IL-1 beta /IL-1F2 by Immunohistochemistry

Oral live L. plantarum GKD7 reduced key proinflammatory cytokines in OA cartilage. (A) IHC staining of IL-1 beta and TNF-alpha expression in representative cartilage from controls, ACLT-only rats, and ACLT + L. plantarum GKD7 rats. Quantitative analyses of (B) IL-1 beta and (C) TNF-alpha in cartilage. Scale bar = 100 µm. * p < 0.05 vs. controls; # p < 0.05 vs. the ACLT-only group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35956346), licensed under a CC-BY license. Not internally tested by R&D Systems.
Human IL-1 beta / IL-1F2 ELISA Standard Curve

Human IL-1 beta / IL-1F2 ELISA Standard Curve

Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) was serially diluted and captured by Mouse Anti-Human IL‑1 beta /IL‑1F2 Monoclonal Antibody (Catalog # MAB601) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-201-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-1 beta/IL-1F2 Antibody (2805)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and THP-1 human acute monocytic leukemia cell line untreated and treated with PMA and LPS

Simple Western

10 µg/mL
Sample: TF‑1 human erythroleukemic cell line

Western Blot

1 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line treated with PMA and LPS

Neutralization

Measured by its ability to neutralize IL‑1 beta /IL‑1F2-induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.05-0.2 µg/mL in the presence of 0.05 ng/mL Recombinant Human IL‑1 beta /IL‑1F2.

Human IL-1 beta /IL-1F2 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL‑1 beta /IL‑1F2 Biotinylated Antibody (Catalog # BAF201)
  • Standard: Recombinant Human IL-1 beta/IL-1F2 Protein (Catalog # 201-LB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 5 reviews rated 4 using MAB601 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

Additional IL-1 beta/IL-1F2 Products

Product Documents for Human IL-1 beta/IL-1F2 Antibody (2805)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-1 beta/IL-1F2 Antibody (2805)

This product is covered by one or more patents, including US Patent # 5,681,933.

For research use only

Citations for Human IL-1 beta/IL-1F2 Antibody (2805)

Customer Reviews for Human IL-1 beta/IL-1F2 Antibody (2805) (5)

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  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: microglial cells
    Species: Human
    Verified Customer | Posted 08/12/2021
    Human IL-1 beta/IL-1F2 Antibody (2805) MAB601
  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human IL-1 beta/IL-1F2 Antibody (2805) MAB601
  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human IL-1 beta /IL-1F2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: head and neck squamous cell carcinoma cells
    Species: Human
    Verified Customer | Posted 09/21/2018
    Human HNSCC cell lines FaDu and HN5 were treated with DMOG for 24 hours and the expression of IL-1beta was detected by western blot.
    Human IL-1 beta/IL-1F2 Antibody (2805) MAB601

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human IL-1 beta/IL-1F2 Antibody (2805)

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  • Q: Can pro-IL-1 beta be detected by Catalog #s AF-201-NA and MAB601 ?

    A: The amino acid sequence present in mature IL-1 beta (Ala117-Ser269) is also present in pro IL-1 beta. So in theory, both Catalog #s AF-201-NA and MAB601 should detect the pro form of IL-1 beta. The results would depend on whether the pro form is abundantly present in the sample type being evaluated.

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