Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # Hu110
View all IL-1 beta/IL-1F2 Primary Antibodies »

Product Specifications

Immunogen

Human IL‑1 beta /IL‑1F2

Specificity

Detects Human IL‑1 beta /IL‑1F2 in direct ELISAs. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Canakinumab. This product is for research use only.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images

Detection of IL-1 beta / IL-1F2 antibody in Human blood monocytes antibody by Flow Cytometry.

Detection of IL-1 beta / IL-1F2 in Human blood monocytes by Flow Cytometry.

Human peripheral blood monocytes treated with 500 ng/mL LPS overnight, were either (A) stained or (B) not stained with Human anti-Human IL-1 beta/IL-1F2 (Research Grade Canakinumab Biosimilar) Antibody (Catalog # MAB10349) followed by APC-conjugated anti-Human IgG secondary antibody (F0135) and Mouse anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Applications

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Human blood monocytes treated with LPS fixed with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (Catalog # FC012)

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

 Canakinumab is a biosimilar directed against human IL-1 beta.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

Additional IL-1 beta/IL-1F2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices

For research use only

Citations for Human IL‑1 beta /IL‑1F2 (Research Grade Canakinumab Biosimilar) Antibody

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FAQs

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  • Q: Does this Biosimilar antibody have the same sequence as the therapeutic antibody?

    A: The Biosimilar antibody has the same variable region and constant region sequence as the therapeutic antibody, except for Catalog numbers MAB9677, MAB9575, MAB9577, MAB9589, and their conjugates. These four antibodies have the same variable region as the Biosimilar, but not the same constant region.

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