IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% aa sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL-29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.
Human IL‑29/IFN‑ lambda 1 Antibody
R&D Systems | Catalog # MAB15981
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
Neutralization, Flow Cytometry, Bioassay, ELISA Capture, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 247801
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-29/IFN-lambda 1
Gly20-Thr200
Accession # Q8IU54
Gly20-Thr200
Accession # Q8IU54
Specificity
Detects human IL-29/IFN-lambda 1 in ELISAs. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) IL-28B/IFN-lambda 3 and 20% with rhIL‑28A is observed. No cross-reactivity or interference was observed with rhIFN-alpha A, rhIFN-alpha B2, rhIFN-alpha G, rhIFN-alpha I, or rhIFN‑ beta 1A.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL‑29/IFN‑ lambda 1 Antibody
IL‑29/IFN‑ lambda 1 Inhibition of EMCV‑induced Cytopathy and Neutralization by Human IL‑29/IFN‑ lambda 1 Antibody.
Recombinant Human IL-29/IFN-lambda 1 (Catalog # 1598-IL) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IL-29/IFN-lambda 1 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anri-Human IL-29/IFN-lambda 1 Monoclonal Antibody (Catalog # MAB15981). The ND50 is typically 1-4 µg/mL.Applications for Human IL‑29/IFN‑ lambda 1 Antibody
Application
Recommended Usage
Neutralization
Measured by its ability to neutralize IL‑29/IFN‑ lambda 1 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑29/IFN‑ lambda 1.
IL-29/IFN-lambda 1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB15981 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-29/IFN-lambda 1
References
- Vilcek, J. (2003) Nature Immunol. 4:8.
- Sheppard, P. et al. (2003) Nature Immunol. 4:63.
- Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
Long Name
Interleukin 29
Alternate Names
IFN-lambda 1, IL29
Entrez Gene IDs
282618 (Human)
Gene Symbol
IFNL1
UniProt
Additional IL-29/IFN-lambda 1 Products
Product Documents for Human IL‑29/IFN‑ lambda 1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑29/IFN‑ lambda 1 Antibody
For research use only
Related Research Areas
Citations for Human IL‑29/IFN‑ lambda 1 Antibody
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Customer Reviews for Human IL‑29/IFN‑ lambda 1 Antibody (2)
4.5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 09/12/2022
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Application: ELISASample Tested: SerumSpecies: HumanVerified Customer | Posted 06/01/2018This antibody was used as the capture in a sandwich ELISA with BAF1598 as the detection.
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