Human IL-29/IFN-lambda 1 Antibody [Biotin]

R&D Systems | Catalog # BAF1598

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Detection, ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all IL-29/IFN-lambda 1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑29/IFN‑ lambda 1
Gly20-Thr200
Accession # Q8IU54

Specificity

Detects human IL‑29/IFN‑ lambda 1 in ELISAs and Western blots. In sandwich immunoassays, approximately 100% cross-reactivity with recombinant human (rh) IL-28B is observed, 35% cross-reactivity with rhIL-28A is observed and less than 0.2% cross‑reactivity with rhIFN‑ alpha A, rhIFN-alpha B2, rhIFN‑ alpha G, rhIFN alpha I, and rhIFN-beta 1A is observed. In Western blots, approximately 25% cross-reactivity with rhIL-28A and rhIL‑28B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IL-29/IFN-lambda 1 Antibody [Biotin]

Human IL-29 / IL-28B (IFN-lambda 1 / 3) ELISA Standard Curve

Human IL-29 / IL-28B (IFN-lambda 1 / 3) ELISA Standard Curve

Recombinant Human IL‑29/IFN‑ lambda 1 (Catalog # 1598-IL) was serially diluted and captured by Mouse Anti-Human IL‑29/IFN‑ lambda 1 Monoclonal Antibody (Catalog # MAB15981) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑29/IFN‑ lambda 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1598) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human IL-29/IFN-lambda 1 Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human IL‑29/IFN-lambda 1 (Catalog # 1598-IL)

IL-29/IFN-lambda 1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human IL‑29/IFN‑ lambda 1 Antibody (Catalog # MAB15981)
  • Standard: Recombinant Human IL-29/IFN-lambda 1 Protein (Catalog # 1598-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using BAF1598 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IL-29/IFN-lambda 1

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11-13% aa sequence identity) and the type I IFN family (15-19% aa sequence identity) (1-3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon, The expression of IL-28A, B, and IL‑29 is induced by virus infection or double-stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor  beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN-regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory region of the target genes. Human IL-29 cDNA encodes a 200 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 181 aa mature protein, which is a monomer in solution. It shares 67% and 69% aa sequence identity with human IL-28A and IL-28B, respectively.

References

  1. Vilcek, J. (2003) Nature Immunol. 4:8.
  2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
  3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.

Long Name

Interleukin 29

Alternate Names

IFN-lambda 1, IL29

Entrez Gene IDs

282618 (Human)

Gene Symbol

IFNL1

UniProt

Q8IU54

Additional IL-29/IFN-lambda 1 Products

Product Documents for Human IL-29/IFN-lambda 1 Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IL-29/IFN-lambda 1 Antibody [Biotin]

For research use only

Related Research Areas

Interferons

Citations for Human IL-29/IFN-lambda 1 Antibody [Biotin]

Customer Reviews for Human IL-29/IFN-lambda 1 Antibody [Biotin] (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human IL-29/IFN-lambda 1 Antibody [Biotin]?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 的 2 reviews Showing All
Filter By:
Clear Filters X
  • Human IL-29/IFN-lambda 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 09/12/2022
    Human IL-29/IFN-lambda 1 Antibody [Biotin] BAF1598
  • Human IL-29/IFN-lambda 1 Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 06/01/2018
    This antibody served as the detection in a sandwich ELISA with MAB15981 as the capture.
    Human IL-29/IFN-lambda 1 Antibody [Biotin] BAF1598

There are no reviews that match your criteria.

Showing  1 - 2 的 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Toll-Like Receptor Signaling Pathways
Toll-Like Receptor Signaling Pathway Thumbnail