Human IL-6R alpha Antibody (17506)

R&D Systems | Catalog # MAB227

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry, Bioassay, ELISA Development, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 17506
View all IL-6R alpha Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha
Leu20-Asp339
Accession # P08887

Specificity

Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-6R alpha Antibody (17506)

Detection of Recombinant Human IL-6R alpha antibody by Western Blot.

Detection of Recombinant Human IL-6R alpha by Western Blot.

Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of IL-6R alpha antibody in U937 Human Cell Line antibody by Flow Cytometry.

Detection of IL-6R alpha in U937 Human Cell Line by Flow Cytometry.

U937 human histiocytic lymphoma cell line was labeled with Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).
IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody.

IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody.

Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND50 is typically 0.04-0.2 µg/mL.
Human IL-6 R alpha ELISA Standard Curve

Human IL-6 R alpha ELISA Standard Curve

Recombinant Human IL-6R alpha (Catalog # 227-SR) was serially diluted and captured by Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of IL-6R alpha by Western Blot

Detection of IL-6R alpha by Western Blot

gp130 and IL-6Ra expression in SCC-4 cells and DOK cells. DOK and SCC-4 cells were untreated or treated with rhIL-6 (30 ng/ml) for 24 h. Lysates were collected and samples were run on 8% SDS-PAGE gel. Western Blot were probed with anti-gp130 and anti-IL-6Ra antibody with anti-beta -actin used as a loading control. Experiments was done in triplicate. Densitometric analysis was performed using image lab software on gp130, IL-6Ra and beta -actin blots. Sample blots for (A) DOK and (B) SCC-4 and collated densitometry data for (Fig. S5A) gp130 and (Fig. S5B) IL-6Ra are presented. Results were plotted using Graphpad prism 8. Values represent the mean ± S.E.M of three independent experiments. Statistical analysis was performed using one-way ANOVA with a post hoc Sidak’s test to compare mean values among the groups, *p < 0.05; **p < 0.01; ***p < 0.001. Western blot figures represent one representative result Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.1007/s12032-022-01664-5), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6R alpha by Western Blot

Detection of IL-6R alpha by Western Blot

SPON1 plays important roles in IL‐6 trans‐signalling. (A) Relative IL‐6 protein levels in SPON1KD cells were measured by ELISA (n = 3). (B) Immunoblot analysis of the indicated cells treated with 5 μM or 10 μM F‐spondin. (C) sIL‐6R and mIL‐6R protein levels in SPON1KD cells were measured by western blotting. (D) Relative numbers of PDAC cells treated with vehicle, 10 μg/ml F‐spondin, 20 μg/ml sgp130 or F‐spondin plus sgp130. (E) EdU assays showed that SPON1 counteracted sgp130‐induced suppression of cell proliferation Scale bar, 50 μm. (F) Immunofluorescence staining for P‐STAT3 (green) in PANC‐1 and SW1990 cells upon indicated treatment. Scale bar, 50 μm. n.s. >0.05 (ANOVA for A; two‐tailed Student t test for D) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35487760), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of IL-6R alpha by Western Blot

Detection of IL-6R alpha by Western Blot

SPON1 plays important roles in IL‐6 trans‐signalling. (A) Relative IL‐6 protein levels in SPON1KD cells were measured by ELISA (n = 3). (B) Immunoblot analysis of the indicated cells treated with 5 μM or 10 μM F‐spondin. (C) sIL‐6R and mIL‐6R protein levels in SPON1KD cells were measured by western blotting. (D) Relative numbers of PDAC cells treated with vehicle, 10 μg/ml F‐spondin, 20 μg/ml sgp130 or F‐spondin plus sgp130. (E) EdU assays showed that SPON1 counteracted sgp130‐induced suppression of cell proliferation Scale bar, 50 μm. (F) Immunofluorescence staining for P‐STAT3 (green) in PANC‐1 and SW1990 cells upon indicated treatment. Scale bar, 50 μm. n.s. >0.05 (ANOVA for A; two‐tailed Student t test for D) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35487760), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-6R alpha Antibody (17506)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑6 R alpha (Catalog # 227-SR)

Neutralization

Measured by its ability to neutralize IL-6R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND50) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Human IL-6R alpha Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IL-6R alpha Biotinylated Antibody (Catalog # BAF227)
  • Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 8 reviews rated 4.8 using MAB227 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References

  1. Yamasaki et al. (1988) Science 241:825.
  2. Baumann et al. (1990) J. Biol. Chem. 265:19853.
  3. Hibi et al. (1990) Cell 63:1149.
  4. Schooltink et al. (1991) Eur. J. Biochem. 277:659.
  5. Novick et al. (1989) J. Exp. Med. 170:1409.
  6. Yasukawa et al. (1990) J. Biochem. 108:673.
  7. Saito et al. (1991) J. Immunology 147:168.

Long Name

Interleukin 6 Receptor alpha

Alternate Names

CD126, IL-6 R alpha, IL-6Ra, IL6R, IL6R alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Gene Symbol

IL6R

UniProt

P08887

Additional IL-6R alpha Products

Product Documents for Human IL-6R alpha Antibody (17506)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IL-6R alpha Antibody (17506)

For research use only

Citations for Human IL-6R alpha Antibody (17506)

Customer Reviews for Human IL-6R alpha Antibody (17506) (8)

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Showing  1 - 5 的 8 reviews Showing All
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  • Human IL-6R alpha Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: DU145 human prostate carcinoma cell line
    Species: Human
    Verified Customer | Posted 12/12/2025
    Blocked with GP130 and IL-6R aplha then stimulated with IL-6
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6R alpha Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 06/02/2025
    We assessed the performance of an anti-hIL-6R monoclonal antibody in a cell-based neutralization assay targeting IL-6 signaling. The assay was designed to measure inhibition of IL-6–induced STAT3 phosphorylation in HEK293 cells expressing gp130 and IL-6R.
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Spleen tissue
    Species: Human
    Verified Customer | Posted 04/03/2023
    Western blot was performed on human spleen tissue using 1 µg/mL of Human IL-6R alpha Antibody.
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6R alpha Antibody
    Name: Katie Myers
    Application: ELISA
    Sample Tested: Serum
    Species: Primate
    Verified Customer | Posted 11/28/2022
    Worked as an ELISA capture antibody
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Human liver cancer cell line (HuH7) and MDA-MB-231
    Species: Human
    Verified Customer | Posted 04/04/2020
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6 R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line
    Species: Human
    Verified Customer | Posted 10/17/2018
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6 R alpha Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 05/31/2018
    Paired with BAF227 this antibody was used to measure IL-6 receptor in human plasma samples using a sandwich immunoassay.
    Human IL-6R alpha Antibody (17506) MAB227
  • Human IL-6 R alpha Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)
    Species: Human
    Verified Customer | Posted 11/29/2017

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