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Human Integrin  alpha 5/CD49e Antibody

Catalog #: AF1864
5 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1864
AF1864-SP

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Detection of Human Integrin a5/CD49e by Western Blot.
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  alpha 5/CD49e Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 5/CD49e in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross‑reactivity with recombinant mouse (rm) Integrin  alpha 5 and less than 5% cross-reactivity with recombinant human Integrin  alpha M, rmIntegrin  alpha 2, rmIntegrin  alpha 4, and rmIntegrin  alpha E is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin  alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Exosome Standard (U87 MG) (Catalog # NBP2-49844), Exosome Standard (PC-3) (Catalog # NBP2-49856), Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Flow Cytometry
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
Adhesion Blockade
The adhesion of K562 human chronic myelogenous leukemia cells (5 x 104 cells/well) to immobilized bovine Fibronectin (Catalog # 1030-FN, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 20 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Integrin a5/CD49e antibody by Western Blot. View Larger

Detection of Human Integrin a5/CD49e by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Integrin a5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Integrin a5/CD49e at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western View Larger

Detection of Human Integrin  alpha 5/CD49e by Simple WesternTM. Simple Western shows lysates of Exosome Standards (U87 MG) (NBP2-49844), Exosome Standards (PC‑3) (NBP2-49856) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Integrin  alpha 5/CD49e at approximately 165 kDa (as indicated) using 20 µg/mL of Goat Anti-Human Integrin  alpha 5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Simple Western View Larger

Detection of Human Integrin  alpha 5/CD49e by Simple WesternTM. Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Integrin  alpha 5/CD49e at approximately 165 kDa (as indicated) using 50 µg/mL of Goat Anti-Human Integrin  alpha 5/CD49e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1864). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 5/CD49e

The alpha 5 subunit, also known as CD49e and VLA-5 alpha subunit, forms a heterodimer with the Integrin beta 1 subunit (CD29). Integrin alpha 5 beta 1 is a receptor for fibronectin and neural adhesion molecule L1.

Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Citations for Human Integrin  alpha 5/CD49e Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Induction of cyclophilin A by influenza A virus infection facilitates group A Streptococcus coinfection
    Authors: X Bai, W Yang, X Luan, H Li, H Li, D Tian, W Fan, J Li, B Wang, W Liu, L Sun
    Cell Reports, 2021-05-18;35(7):109159.
    Species: Human
    Sample Types: Whole Cells
    Applications: Co-Immunoprecipitation, ICC
  2. Preparation of single- and double-oligonucleotide antibody conjugates and their application for protein analytics
    Authors: J Wiener, D Kokotek, S Rosowski, H Lickert, M Meier
    Sci Rep, 2020-01-29;10(1):1457.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: ELISA Capture
  3. Macrophage-secreted factors promote a profibrotic phenotype in human preadipocytes.
    Authors: Keophiphath M, Achard V, Henegar C, Rouault C, Clement K, Lacasa D
    Mol. Endocrinol., 2008-10-22;23(1):11-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. IFATS collection: Combinatorial peptides identify alpha5beta1 integrin as a receptor for the matricellular protein SPARC on adipose stromal cells.
    Authors: Nie J, Chang B, Traktuev DO, Sun J, March K, Chan L, Sage EH, Pasqualini R, Arap W, Kolonin MG
    Stem Cells, 2008-06-26;26(10):2735-45.
    Species: Human
    Sample Types: Proteins
    Applications: Western Blot
  5. Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture.
    Authors: Page JL, Johnson MC, Olsavsky KM
    Toxicol. Sci., 2007-02-27;97(2):384-97.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human Integrin  alpha 5/CD49e Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human Integrin  alpha 5/CD49e Antibody
By Anonymous on 04/25/2018
Application: WB Sample Tested: HMVEC human microvascular endothelial cells Species: Rabbit
Human Integrin  alpha 5/CD49e Antibody
By Anonymous on 04/24/2018
Application: WB Sample Tested: blood endothelial cells Species: Human

Used at concentration of 1 in 2000
Band seen at around 160kDa
Worked in both reducing and non-reducing conditions
Would use again

Western Blot Human Integrin  alpha 5/CD49e Antibody AF1864