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Human Integrin  alpha 5/CD49e PE-conjugated Antibody

Catalog #: FAB1864P
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1864P

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Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.
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Citations (3)
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Human Integrin  alpha 5/CD49e PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin alpha 5/CD49e.
Source
Monoclonal Mouse IgG1 Clone # 238307
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin alpha 5/CD49e
Phe42-Tyr995
Accession # P08648
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin a5/CD49e antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin a5/CD49e PE-conjugated Monoclonal Antibody (Catalog # FAB1864P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 5/CD49e

The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).

References
  1. Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
  2. Te Velde, A. et al. (1988) J. Immunol. 140:1548.
Entrez Gene IDs
3678 (Human); 16402 (Mouse); 102114969 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5

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Citations for Human Integrin  alpha 5/CD49e PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Decidua Parietalis Mesenchymal Stem/Stromal Cells and Their Secretome Diminish the Oncogenic Properties of MDA231 Cells In Vitro
    Authors: Y Basmaeil, E Bahattab, A Al Subayyi, HB Kulayb, M Alrodayyan, M Abumaree, T Khatlani
    Cells, 2021-12-10;10(12):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Conditions Mimicking the Cancer Microenvironment Modulate the Functional Outcome of Human Chorionic Villus Mesenchymal Stem/Stromal Cells in vitro
    Authors: Yasser Basmaeil, Abdullah Al Subayyil, Mohammad Abumaree, Tanvir Khatlani
    Frontiers in Cell and Developmental Biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Increased numbers of immature plasma cells in peripheral blood specifically overexpress chemokine receptor CXCR3 and CXCR4 in patients with ulcerative colitis.
    Authors: Hosomi S, Oshitani N, Kamata N, Sogawa M, Okazaki H, Tanigawa T, Yamagami H, Watanabe K, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Clin. Exp. Immunol., 2010-11-19;163(2):215-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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