Home / Integrin beta 8 / Human Integrin  beta 8 Antibody

Human Integrin  beta 8 Antibody

Catalog #: MAB4775
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4775
MAB4775-SP

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Detection of Integrin beta 8 in A172 Human Cell Line by Flow Cytometry.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Integrin  beta 8 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Integrin beta 8 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Integrins beta 1, beta 2, beta 3, beta 4, beta 5, beta 6, beta 7, or recombinant mouse Integrin beta 8 is observed.
Source
Monoclonal Mouse IgG2B Clone # 416922
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Integrin beta 8
Glu43-Arg684 (predicted)
Accession # P26012
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 8 antibody in A172 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 8 in A172 Human Cell Line by Flow Cytometry. WM-115 human melanoma cell line was stained with Human Integrin beta 8 Monoclonal Antibody (Catalog # MAB4775, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 8

Integrin beta 8 (Integrin beta 8) is a 90 kDa type I transmembrane glycoprotein of the Integrin family of adhesion molecules. It associates with Integrin alpha V to form a receptor for vitronectin, fibrin, and the latency associated peptide (LAP). Binding to LAP promotes the proteolytic release of active TGF-beta from LAP. Integrin alpha V beta 8 is required for vascular morphogenesis in the embryonic brain and yolk sac. Within the extracellular domain, human Integrin  beta 8 shares 87% aa sequence identity with mouse and rat Integrin  beta 8.

Entrez Gene IDs
3696 (Human); 320910 (Mouse); 362800 (Rat)
Alternate Names
Integrin beta 8; integrin beta-8; integrin, beta 8; ITGB8

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Citations for Human Integrin  beta 8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes
    Authors: Rouaud, L;Baudin, L;Gautier-Isola, M;Van Meerbeeck, P;Feyereisen, E;Blacher, S;van Baren, N;Kridelka, F;Lucas, S;Noel, A;
    Cancers
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Integrin-alphaV-mediated activation of TGF-beta regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade
    Authors: I Malenica, J Adam, S Corgnac, L Mezquita, E Auclin, I Damei, L Grynszpan, G Gros, V de Montpré, D Planchard, N Théret, B Besse, F Mami-Choua
    Nature Communications, 2021-09-01;12(1):5209.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Highly invasive melanoma cells activate the vascular endothelium via an MMP-2/integrin alphavbeta5-induced secretion of VEGF-A.
    Am. J. Pathol., 2012-05-31;181(2):693-705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Slow-Cycling Cells in Glioblastoma: A Specific Population in the Cellular Mosaic of Cancer Stem Cells
    Authors: Changlin Yang, Guimei Tian, Mariana Dajac, Andria Doty, Shu Wang, Ji-Hyun Lee et al.
    Cancers (Basel)

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