Human IRF8 Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB5117U Datasheet / COA / SDS

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FAB5117U-100UG

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Human IRF8 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IRF8 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IRF4 is observed.

Source

Monoclonal Mouse IgG2a Clone # 809926

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human IRF8
Ala255-Val426
Accession # Q02556

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRF8

IRF8 (interferon regulatory factor 8; also ICSBP) is a 50 kDa member of the IRF family of proteins. It is expressed in myeloid, dendritic and B cells and interacts with PU.1 to regulate the expression of TLR4, IL-18, IL-12p35, and PTPN13, a Fas-associated phosphatase that blocks Fas-induced apoptosis. Human IRF8 is 426 amino acids (aa) in length. It contains an N-terminal DNA-binding basic region (aa 9-110) and at least one regulatory phosphorylation site at Tyr95. Over aa 255-426, human IRF8 shares 90% and 88% aa sequence identity with canine and mouse IRF8, respectively.

Long Name

Interferon Regulatory Factor 8

Entrez Gene IDs

3394 (Human); 15900 (Mouse); 292060 (Rat)

Alternate Names

ICSBP1; ICSBPH-ICSBP; interferon consensus sequence binding protein 1; Interferon consensus sequence-binding protein; interferon regulatory factor 8; IRF8; IRF-8ICSBP1