Human Irisin/FNDC5 Alexa Fluor™ Plus 594‑conjugated Antibody

R&D Systems | Catalog # FAB94202AFP594

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Alexa Fluor Plus 594 (Excitation = 590 nm, Emission = 618 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 1056126
View all Irisin/FNDC5 Primary Antibodies »

Product Specifications

Specificity

Detects Irisin peptide in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Irisin/FNDC5 Alexa Fluor™ Plus 594‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Irisin/FNDC5

Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development (1, 2). Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation (2-5). Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise (2, 6). Irisin induces expression of peroxisome proliferator-activated receptor  gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins (7, 8). Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes (1, 2). A similar function in humans is suggested (9). Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts (10-14).

References

  1. Bostrom, P. et al. (2012) Nature 481:463.
  2. Novelle, M.G. et al. (2013) Int. J. Endocrinol. 2013:746281.
  3. Ferrer-Martinez, A. et al. (2002) Dev. Dyn. 224:154.
  4. Teufel, A. et al. (2002) Gene 297:79.
  5. Zhang, W. et al. (2015) Neurosci. Lett. 595:7.
  6. Roca-Rivada, A. et al. (2013) PLoS One 8:e60563.
  7. Vaughan, R.A. et al. (2014) Diabetes Obes. Metab. 16:711.
  8. Jeong Lee, H. et al. (2015) Mol. Endocrinol. 6:873.
  9. Elsen, M. et al. (2014) J. Endocrinol. 222:R25.
  10. Dun, S.L. et al. (2013) Neuroscience 240:155.
  11. Forouzanfar, M. et al. (2015) Cell. Biol. Int. 39:629.
  12. Hashemi, M.S. et al. (2013) Neuroscience 231:296.
  13. Moon, H.S. et al. (2013) Metabolism 62:1131.
  14. Colaianni, G. et al. (2014) Int. J. Endocrinol. 2014:902186.

Long Name

Fibronectin Type III Domain Containing 5

Alternate Names

FNDC5, FRCP2

Entrez Gene IDs

252995 (Human); 384061 (Mouse); 260327 (Rat)

Gene Symbol

FNDC5

UniProt

Q8NAU1

Additional Irisin/FNDC5 Products

Product Documents for Human Irisin/FNDC5 Alexa Fluor™ Plus 594‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Irisin/FNDC5 Alexa Fluor™ Plus 594‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Diabetes
Obesity

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Protocols

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