Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 945842
View all LIF Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived human LIF
Met1-Phe202
Accession # P15018

Specificity

Detects human LIF in direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human LIF Antibody

Human LIF ELISA Standard Curve.

Recombinant Human LIF protein was serially diluted 2-fold and captured by Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB2502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human LIF Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human LIF DuoSet ELISA Kit (Catalog # DY7734-05) for convenient development of a sandwich ELISA.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

LIF is a 36‑67 kDa highly glycosylated polypeptide (1, 2) produced by a variety of cells including T cells (3), monocytes (4), fibroblasts (5), osteoblasts (6) and mast cells (7). Consistent with its many synonyms, LIF exhibits a broad spectrum of effects on both hematopoietic and nonhematopoietic cells. For example, LIF inhibits the differentiation of embryonic stem cells (8), up regulates the synthesis of acute phase proteins in hepatocytes (9), down regulates lipoprotein lipase activity in adipocytes (10), and preferentially induces a cholinergic phenotype in sympathetic neurons (11). The receptor for LIF (LIF R) has been isolated and found to be a 190 kDa type I transmembrane glycoprotein (12). Although this molecule binds LIF, the resultant LIF-LIF R complex is not sufficient to transduce an intracellular signal. This capability is provided by a 130 kDa signal transducing subunit (gp130) that is common to the functional receptors for IL-6, IL-11, CNTF, and Oncostatin M (13, 14). Since gp130 is a ubiquitously expressed membrane protein, the presence of LIF R (membrane-bound or soluble form) ultimately determines the cell’s responsiveness to LIF. Cells known to express LIF R include osteoblasts (6), hepatocytes (15), macrophages (15), neurons (5), and megakaryocytes (16). Human and mouse LIF exhibit 78% sequence homology, and human LIF is biologically active on mouse cells.

References

  1. Van-Vlasselaev, P. et al. (1992) Prog. Growth Factor Res. 4:337.
  2. Gough, N.M. (1992) Growth Factors 7:175.
  3. Anegon, N.M, I. et al. (1991) J. Immunol.147:3973.
  4. Gillett, N.A. et al. (1993) Growth Factors 9:301.
  5. Banner, L. R. and P.H. Patterson (1994) Proc. Natl. Acad. Sci. 91:7109.
  6. Allen, E.H. et al. (1990) J. Cell. Physiol. 145:110.
  7. Lorenzo, J.A. et al. (1994) Clin.Immunol. Immunopathol. 70:260.
  8. Williams, R.L. et al. (1988) Nature 336:684.
  9. Baumann, H. et al. (1989) J. Immunol. 143:1163.
  10. Marshall, M.K. et al. (1994) Endocrinology 135:1412.
  11. Ludham, W.H. et al. (1994) Dev. Biol. 164:5283.
  12. Davis, S. et al. (1993) Science 260:18054.
  13. Gearing, D.P. et al. (1991) EMBO J. 10:28395.
  14. Gearing, D.P. et al. (1991) Science 255:1434.
  15. Hilton, D.J. and N.A. Nicola (1992) J. Biol. Chem. 267:102286.
  16. Hilton, D.J. et al. (1992) Ciba17. Foundation Symposium 167:2278.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

UniProt

P15018

Additional LIF Products

Product Documents for Human LIF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIF Antibody

For research use only

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