M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
ELISA Capture, Luminex Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 21113
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Product Specifications
Immunogen
E. coli-derived recombinant human M-CSF
Specificity
Detects recombinant human (rh)M-CSF in Western blots. In sandwich ELISAs, less than 7% cross-reactivity was observed with rmM-CSF. No significant cross-reactivity or interference was observed with over 75 other cytokines tested.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human M-CSF Antibody (21113)
Human M-CSF ELISA Standard Curve
Recombinant Human M‑CSF (Catalog # 216-MC) was serially diluted and captured by Mouse Anti-Human M‑CSF Monoclonal Antibody (Catalog # MAB616) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human M‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF216) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human M-CSF Antibody (21113)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only
Sample: Recombinant Human M-CSF (Catalog # 216-MC)
under non-reducing conditions only
Human M-CSF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 5 using MAB616 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: M-CSF
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
Additional M-CSF Products
Product Documents for Human M-CSF Antibody (21113)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human M-CSF Antibody (21113)
For research use only
Citations for Human M-CSF Antibody (21113)
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Customer Reviews for Human M-CSF Antibody (21113) (2)
5 out of 5
2 Customer Ratings
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Application: ELISASample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 07/23/2019
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 11/03/2017This antibody was used as a capture antibody in the development of a sandwich ELISA. The R&D systems polyclonal antibody AF216 was used as the detection antibody. M-CSF was measured in human serum samples with this assay.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
