Human MELK Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4820S Datasheet / COA / SDS

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AF4820S-100UG

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Human MELK Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human MELK in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MELK
Gln341-Ala470
Accession # Q14680

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MELK

MELK (maternal embryonic leucine zipper kinase; also HPK38) is a member of the Snf1/AMPK family of serine/threonine kinases. It is expressed in blood mononuclear cells, stem cells and other tissues, and functions in cell cycle progression and pre-mRNA splicing. Human MELK is 651 amino acids (aa) in length and contains one protein kinase domain (aa 11‑263) and a kinase-associated (KA) 1 domain (aa 602‑651). MELK is activated upon phosphorylation of T167 and S171. There are multiple alternative splice variants. Two show the same 16 aa substitution for the N-terminal 87 and 48 aa, respectively; a third shows an alternate start site at M440, and a fourth shows complex splicing over aa 1‑392. Over aa 341‑470, human MELK shares 56% aa identity with mouse MELK.

Long Name

Maternal Embryonic Leucine Zipper Kinase

Entrez Gene IDs

9833 (Human)

Alternate Names

EC 2.7.11; EC 2.7.11.1; hMELK; HPK38; KIAA0175pEg3 kinase; maternal embryonic leucine zipper kinase; MELK; PK38; Protein kinase PK38