Human Mesothelin Antibody (420404)

R&D Systems | Catalog # MAB32651

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 420404
View all Mesothelin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272

Specificity

Detects human Mesothelin in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Mesothelin Antibody (420404)

Mesothelin antibody in Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

Mesothelin in Human Ovarian Cancer Tissue.

Mesothelin was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 5 µg/mL Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32651) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mesothelin by Immunohistochemistry

Detection of Mesothelin by Immunohistochemistry

Human patients with adhesions have EGFR positive cells of mesothelial origin in them and an elevated EGFR-agonist expression in their peritoneal leukocytes.a Biopsy from a patient with a fresh adhesion (13 days after initial surgery) stained for mesothelin and epidermal growth factor receptor (EGFR). Image depicts omental tissue that was adherent to a piece of intestine (the latter could not be included in the biopsy). Magnification shows cells that are double positive for mesothelin and EGFR. Scale bar overview: 500 um, Scale bar magnification: 50 µm. Images are representative of n = 4 human patients. b–d Peritoneal leukocytes were isolated from patients with no, fresh or old adhesions. Expression of EGFR ligand genes was measured by quantitative polymerase chain reaction. (b) amphiregulin (AREG), none vs. fresh: p = 0.0081, none vs. old: p = 0.96, fresh vs. old: p = 0.0056. c epiregulin (EREG), none vs. fresh: p = 0.016, none vs. old: p = 0.24, fresh vs. old: p = 0.0028. (d) epidermal growth factor (EGF), none vs. fresh: p = 0.028, none vs. old: p = 0.48, fresh vs. old: p = 0.02, Data are represented as mean and individual symbols. Data represent n = 8 for none, 4 for fresh, and 9 for old individual patients of one independent cohort study. Patient demographics according to Supplementary Table 2. Indicated statistical differences by Wilcoxon test (two-sided) with Holm-Bonferroni correction for multiple-testing. *P < 0.05, **P < 0.01, n.s. P ≥ 0.05. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34916513), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Mesothelin Antibody (420404)

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human ovarian cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB32651 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272

Additional Mesothelin Products

Product Documents for Human Mesothelin Antibody (420404)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Antibody (420404)

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Mesothelin Antibody (420404)

Customer Reviews for Human Mesothelin Antibody (420404) (2)

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  • Human Mesothelin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovarian cancer tissue
    Species: Human
    Verified Customer | Posted 11/24/2021
    Human Mesothelin Antibody (420404) MAB32651
  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: See PMID 22705407
    Species: Rat
    Verified Customer | Posted 02/19/2015

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