Human mGluR5 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4514R Datasheet / COA / SDS

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AF4514R-100UG

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Human mGluR5 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human mGluR5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human (rh) mGluR1 is observed and less than 5% cross-reactivity with rhmGluR2 and rhmGluR3

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human mGluR5
Ser19-Ser509
Accession # P41594

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: mGluR5

Human metabotropic glutamate receptor 5 (mGluR5; also mGluR5b) is a 150 kDa, 7-transmembrane glycoprotein that belongs to group I of the C-family of G-protein coupled receptors. mGluR5 is constitutively expressed and regulates neuronal ion channel activity. Human mGluR5 is 1212 amino acids (aa) in length and contains an extracellular domain (ECD) of 558 amino acids. Through its ECD, mGluR5 either homodimerizes or heterodimerizes with the Ca⁺⁺-sensor receptor. There is one alternate splice form (mGluR5a) that shows a 32 aa deletion between aa 877‑908 in the cytoplasmic tail. Over aa 19‑509, human mGluR5 is 98% aa identical to mouse, rat and canine mGluR5.

Long Name

Metabotropic Glutamate Receptor 5

Entrez Gene IDs

2915 (Human); 108071 (Mouse); 24418 (Rat)

Alternate Names

glutamate receptor, metabotropic 5; GPRC1E; GPRC1EMGLUR5metabotropic glutamate receptor 5; GRM5; mGlu5; mGluR5