Human/Mouse Apolipoprotein A-IV/ApoA4 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF8125S Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF8125S-100UG

Save 15% on Select RUO Reagents. See Details.

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Apolipoprotein A-IV/ApoA4 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Apolipoprotein A-IV/ApoA in direct ELISAs. Detects human and mouse in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-dervied recombinant human Apolipoprotein A-IV/ApoA
Glu21-Ser396
Accession # P06727

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Apolipoprotein A-IV/ApoA4

Apolipoprotein A4 (ApoA4) is a 45 kDa glycoprotein of the lipid transport system. Secreted in plasma, ApoA4 is a major component of high density lipoprotein (HDL) particles and chylomicrons, and is thought to act in intestinal lipid absorption. Levels of ApoA4 may influence HDL metabolism and modulate its effects on atherogenesis. ApoA4 synthesis in humans is mainly confined to the small intestine, while in mice and rats, production occurs in the liver as well. Human ApoA4 is synthesized as a 396 amino acid (aa) precursor, from which a 20 aa N-terminal signal peptide is removed. Mature human ApoA4 (aa 21-396) shares 61% and 62% aa sequence identity with mouse and rat ApoA4, respectively.

Entrez Gene IDs

337 (Human); 11808 (Mouse); 25080 (Rat)

Alternate Names

apo-AIV; apoA-IV; Apolipoprotein A4; Apolipoprotein AIV; Apolipoprotein A-IV; MGC142154; MGC142156