Human/Mouse/Bovine Integrin alpha 6/CD49f PE-conjugated Antibody

Catalog #: FAB13501P
5 Citations Datasheet / COA / SDS

Clone GoH3 was used by HLDA to establish CD designation

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FAB13501P

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Detection of Integrin alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry.

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Citations (5)

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Human/Mouse/Bovine Integrin alpha 6/CD49f PE-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Bovine

Specificity

Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.

Source

Monoclonal Rat IgG_2A Clone # GoH3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse mammary tumor cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Integrin alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f PE-conjugated Monoclonal Antibody (Catalog # FAB13501P) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of Integrin alpha 6/CD49f in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f PE-conjugated Monoclonal Antibody (Catalog # FAB13501P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 6/CD49f

The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits and recognizes laminin as a ligand.

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Alternate Names

CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse/Bovine Integrin alpha 6/CD49f PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Combinatorial genetic strategy accelerates the discovery of cancer genotype-phenotype associations
Authors: Li, S;Wong, A;Sun, H;Bhatia, V;Javier, G;Jana, S;Montgomery, RB;Wright, JL;Lam, HM;Hsieh, AC;Faltas, BM;Haffner, MC;Lee, JK;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Human Basal and Suprabasal Keratinocytes Are Both Able to Generate and Maintain Dermo-Epidermal Skin Substitutes in Long-Term In Vivo Experiments
Authors: L Pontiggia, AK Ahuja, HK Yosef, D Rütsche, E Reichmann, U Moehrlen, T Biedermann
Cells, 2022-07-09;11(14):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity
Authors: B Kumar, AK Adebayo, M Prasad, ML Capitano, R Wang, P Bhat-Naksh, M Anjanappa, E Simpson, D Chen, Y Liu, JM Schilder, AB Colter, C Maguire, CJ Temm, G Sandusky, EH Doud, AB Wijeratne, AL Mosley, HE Broxmeyer, H Nakshatri
Science Advances, 2022-01-12;8(2):eabh3375.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnusrhamnoides (L.) A. Nelson-The Implications for Human Barrier Cells
Authors: B Sadowska, J Rywaniak, A Cichocka, K Cichocka, J ?uchowski, U Wójcik-Boj, M Wi?ckowska, B Ró?alska
Molecules, 2020-05-09;25(9):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Partial maintenance and long-term expansion of murine skin epithelial stem cells by Wnt-3a in vitro.
Authors: Ouji Y, Ishizaka S, Nakamura-Uchiyama F, Okuzaki D, Yoshikawa M
J Invest Dermatol, 2014-12-01;135(6):1598-608.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry