Human/Mouse CDK4 Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: CDK4

CDK4 (cyclin-dependent kinase 4) is a 34 kDa member of the CMGC Ser/Thr protein kinase family of enzymes. Within the cell, pRb/retinoblastoma protein is normally unphosphorylated and suppresses cell growth at the G1 stage of the cell cycle. When mitosis is required, CDK4 is phosphorylated, forms a complex with cyclin D1, 2 or 3, and phosphorylates pRb/retinoblastoma protein. This inactivates pRb and cells initiate DNA synthesis. Human CDK4 is 303 amino acids (aa) in length. It contains one protein kinase domain (aa 6‑295) and an activating phosphorylation site at Thr172. There are three potential variants; one that shows an alternate start site at Met75, a second that shows a two aa substitution for aa 89‑211, and a third that shows a 23 aa substitution for aa 89‑303. Full-length human and mouse CDK4 are 95% aa identical.

Product Datasheets

Related Research Areas

• Cell Cycle
• Checkpoint
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Search Kinases and Phosphatases