Human/Mouse Doublecortin/DCX Alexa Fluor® 750-conjugated Antibody

Catalog #: AF10025S Datasheet / COA / SDS

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AF10025S-100UG

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Human/Mouse Doublecortin/DCX Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Doublecortin/DCX in direct ELISAs and Western blots. Detects mouse Doublecortin/DCX in immunohistochemistry.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E.coli-derived recombinant human Doublecortin/DCX
Met116-Thr293
Accession # O43602

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Doublecortin/DCX

Doublecortin (DCX) is a 365 aminoacids, 40 kDa microtubule-associated phosphoprotein (MAP) encoded by the DCX gene. DCX is an essential factor in neurogenesis, modulating, stabilizing and stimulating polymerization of microtubules (MTs) to ensure effective migration of neurons of the central and peripheral nervous system during embryonic and postnatal development. Mutations in this protein impairs neuronal migration leading to several pathological conditions.

DCX is very conserved across species. Human and non-human primates DCX proteins are 98-99% identical at the aminoacid level, and 100% identical at the sequences used for immunogen of AF10025; human, mouse and rat DCX aminoacid sequences are 98% identical.

Long Name

Doublecortin

Entrez Gene IDs

1641 (Human); 13193 (Mouse); 84394 (Rat)

Alternate Names

DBCN; Doublecortex; Doublecortin; Doublin; FLJ51296; Lissencephalin-X; LISX; Lis-X; X-linked (doublecortin)