Human/Mouse Gastrokine 1 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF7287R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7287R-100UG

Save 15% on Select RUO Reagents. See Details.

All Gastrokine 1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Gastrokine 1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse Gastrokine 1 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Gastrokine 1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Gastrokine 1
Tyr38-Tyr201
Accession # Q9CR36

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Gastrokine 1

Gastrokine-1 (GKN1; also CA11 and AMP-18) is an 18-20 kDa member of the Gastrokine protein family. It has limited expression, being restricted to mucous secreting pyloric antrum epithelial cells. Gastrokine-1 appears to promote epithelial cell proliferation and migration, and induce the formation of tight junctions between epithelial cells. By contrast, gastrokine-1 induces Fas expression in tumor cells, resulting in apoptosis. Mature mouse gastrokine-1 is 166 amino acids (aa) in length (aa 36-201). Based on the SwissProt sequence, it possesses one BRICHOS domain (aa 71-165) that contains a mitogenic sequence (aa 113-131). There is one potential alternative start site at Met18. Over aa 38-201, mouse gastrokine-1 shares 65% and 92% aa sequence identity with human and rat gastrokine-1, respectively.

Long Name

Take out Column D name

Entrez Gene IDs

56287 (Human); 66283 (Mouse); 297418 (Rat)

Alternate Names

18 kDa antrum mucosa protein; AMP18; AMP-18; AMP18BRICHOS domain containing 1; BRICD1; CA11; CA11MGC70354; FOV; Foveolin; Gastrokine 1; gastrokine-1; GKN1; Protein CA11