HMGB1/HMG-1 Antibody (1002147)

R&D Systems | Catalog # MAB16902

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1002147
View all HMGB1/HMG-1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human HMGB1
Thr85-Gly166
Accession # P09429

Specificity

Detects human HMGB1 in direct ELISAs. Detects human and mouse HMGB1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for HMGB1/HMG-1 Antibody (1002147)

HMGB1/HMG-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

HMGB1/HMG‑1 in HeLa Human Cell Line.

HMGB1/HMG-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse HMGB1/HMG-1 Monoclonal Antibody (Catalog # MAB16902) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for HMGB1/HMG-1 Antibody (1002147)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB16902 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HMGB1/HMG-1

Human High-mobility group box 1 protein (HMGB1), previously known as HMG-1 or amphoterin, is a member of the high mobility group box family of non-histone chromosomal proteins (1‑3). Human HMGB1 is expressed as a 30 kDa, 215 amino acid (aa) single chain polypeptide containing three domains: two N-terminal globular, 70 aa positively charged DNA-binding domains (HMG boxes A and B), and a negatively charged 30 aa C-terminal region that contains only Asp and Glu (4, 5). Residues 27‑43 and 178‑184 contain a NLS. Posttranslational modifications of the molecule have been reported, with acetylation occurring on as many as 17 lysine residues (6). HMGB1 is expressed at high levels in almost all cells (2, 4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1, 7, 8). It is now known that HMGB1 can also act extracellularly, both as an inflammatory mediator that promotes monocyte migration and cytokine secretion, and as a mediator of T cell-dendritic cell interaction (1, 4, 7, 9, 10). The cytokine activity of HBMG1 is restricted to the HMG B box, (3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence, it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6, 12). Once secreted, HMGB1 transduces cellular signals through its high affinity receptor, RAGE and, possibly, TLR2 and TLR4 (1, 3, 4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse, rat, bovine and porcine HMGB1, respectively.

References

  1. Lotze, M.T. and K.J. Tracey (2005) Nat. Rev. Immunol. 5:331.
  2. Yang, H. et al. (2005) J. Leukoc. Biol. 78:1.
  3. Dumitriu, I.E. et al. (2005) Trends Immunol. 26:381.
  4. Degryse, B. and M. de Virgilio (2003) FEBS Lett. 553:11.
  5. Wen, L. et al. (1989) Nucleic Acids Res. 17:1197.
  6. Bonaldi, T. et al. (2003) EMBO J. 22:5551.
  7. Muller, S. et al. (2001) EMBO J. 20:4337.
  8. Bustin, M. (1999) Mol. Cell. Biol. 19:5237.
  9. Wang, H. et al. (1999) Science. 285:248.
  10. Dimitriu, I.E. et al. (2005) J. Immunol. 174:7506.
  11. Najima, Y. et al. (2005) J. Biol. Chem. 280:27523.
  12. Gardella, S. et al. (2002) EMBO Rep. 3:995.

Long Name

High Mobility Group Protein 1

Alternate Names

HMG-1, HMG1

Entrez Gene IDs

3146 (Human)

Gene Symbol

HMGB1

UniProt

P09429

Additional HMGB1/HMG-1 Products

Product Documents for HMGB1/HMG-1 Antibody (1002147)

Certificate of Analysis

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Product Specific Notices for HMGB1/HMG-1 Antibody (1002147)

For research use only

Citations for HMGB1/HMG-1 Antibody (1002147)

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  • Human/Mouse HMGB1/HMG-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: glioma cells
    Species: Mouse
    Verified Customer | Posted 05/26/2022
    HMGB1/HMG-1 Antibody (1002147) MAB16902

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