Human/Mouse Lipoprotein Lipase/LPL Alexa Fluor® 700-conjugated Antibody

Catalog #: AF7197N Datasheet / COA / SDS

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AF7197N-100UG

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Human/Mouse Lipoprotein Lipase/LPL Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Lipoprotein Lipase/LPL in Western blots. Detects human Lipoprotein Lipase/LPL in direct ELISAs and less than 1% cross-reactivity with recombinant human (rh) LIPG, rhLIPI, and rhPNLIPRP1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Lipoprotein Lipase/LPL
Accession # P06858

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Lipoprotein Lipase/LPL

LPL (LipoProtein Lipase; also LIPD) is a 53-56 kDa glycoprotein member of the Lipase family, AB Hydrolase superfamily of molecules. It is produced by multiple cell types, including adipocytes, skelelal muscle cells and macrophages. Once secreted, the circulating enzyme ultimately becomes immobilized on the surface of endothelium by binding to cell surface heparan sulfate. Here, it hydrolyzes triglycerides embedded in chylomicrons and VLDLs by homodimerizing and interacting with apoC2. Mature human LPL is 448 amino acids (aa) in length. It contains an enzymatic region (aa 37-334) plus one protein-interaction PLAT domain (aa 341-465). Over aa 28-154, human LPL shares 91% aa identity with mouse LPL.

Entrez Gene IDs

4023 (Human); 16956 (Mouse); 24539 (Rat)

Alternate Names

EC 3.1.1; HDLCQ11; LIPD; LIPDEC 3.1.1.34; Lipoprotein Lipase; LPL