Human/Mouse Neurturin Alexa Fluor® 647-conjugated Antibody

Catalog #: AF477R Datasheet / COA / SDS

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AF477R-100UG

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Human/Mouse Neurturin Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Neurturin in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Neurturin

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Blockade of Receptor-ligand Interaction

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Neurturin

Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. GDNF family proteins are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1 - 4). Similar to other TGF-beta family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Biologically active Neurturin is a disulfide-linked homodimer of the carboxy-terminal 100 amino acid residues. Mature mouse Neurturin shares 91% amino acid sequence similarity with human Neurturin. Mature Neurturin also shares about 40% similarities with the other three members of the GDNF family ligands (2, 5). Unlike other members of TGF-beta family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFR alpha -1 - GFR alpha -4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFR alpha -2 but can also bind GFR alpha -1 at higher concentration (5 - 8). Neurturin has been shown to promote the survival of a variety of neurons including sympathetic, sensory, and central nervous system neurons. Neurturin is expressed in both neuronal and non-neuronal tissues. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems as well as non-neuronal systems (9).

Entrez Gene IDs

4902 (Human); 18188 (Mouse)

Alternate Names

Neurturin; NRTN; NTN