Human/Mouse PDZK1 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF4997G Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4997G-100UG

Save 15% on Select RUO Reagents. See Details.

All PDZK1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse PDZK1 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse PDZK1.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDZK1
Met1-Met519
Accession # Q5T2W1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PDZK1

PDZK1 (PDZ Domain-containing 1; also CFTR-associated protein of 70 kDa/CAP70) is a 65 - 70 kDa member of the NHER (Na/H Exchange Regulator) family of proteins. It is only expressed in epithelium, particularly that found in intestine, kidney and liver. PDZK1 serves as a scaffold protein that connects membrane proteins to cytoplasmic regulators. Human PDZK1 is 519 amino acids (aa) in length. It contains four PDZ domains (aa 9 - 90; 134 - 215; 243 - 323; and 378 - 458) plus two phosphorylation sites at Ser492 and Ser514. There are two potential truncated forms of human PDZK1, one that ends after Leu330, and a second that ends after Lys264. Human PDZK1 shares 78% aa identity with mouse PDZK1.

Long Name

PDZ Domain Containing 1

Entrez Gene IDs

5174 (Human); 59020 (Mouse); 65144 (Rat)

Alternate Names

CAP70; CFTR-associated protein of 70 kDa; CLAMP; Na(+)/H(+) exchanger regulatory factor 3; Na/Pi cotransporter C-terminal-associated protein 1; NaPi-Cap1; NHERF3; NHERF-3; NHERF3PDZD1Na(+)/H(+) exchange regulatory cofactor NHE-RF3; PDZ domain containing 1; PDZ domain-containing protein 1; PDZ-containing kidney protein 1; PDZD1; PDZK1; Sodium-hydrogen exchanger regulatory factor 3