PRDM16/MEL1 Antibody

R&D Systems | Catalog # AF6295

R&D Systems
Discontinued Product
AF6295 has been discontinued. View all PRDM16/MEL1 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Co-Immunoprecipitation, Screening

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PRDM16/MEL1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse PRDM16
Lys537-Glu688
Accession # A2A935.1

Specificity

Detects mouse and human PRDM16 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for PRDM16/MEL1 Antibody

Detection of Human PRDM16 antibody by Western Blot.

Detection of Human PRDM16 by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
PRDM16 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

PRDM16 in Mouse Embryo.

PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for PRDM16/MEL1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13.5)

Western Blot

1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and M1 mouse myeloid leukemia cell line

Reviewed Applications

Read 1 review rated 3 using AF6295 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PRDM16/MEL1

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

Long Name

PR Domain Containing 16

Alternate Names

MDS1/EVI1-like gene 1, MEL1, PFM13

Entrez Gene IDs

63976 (Human); 70673 (Mouse)

Gene Symbol

PRDM16

UniProt

A2A935.1

Additional PRDM16/MEL1 Products

Product Documents for PRDM16/MEL1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PRDM16/MEL1 Antibody

For research use only

Citations for PRDM16/MEL1 Antibody

Customer Reviews for PRDM16/MEL1 Antibody (1)

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Customer Images

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  • Human/Mouse PRDM16/MEL1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adipose tissue and Brown adipose tissue
    Species: Mouse
    Verified Customer | Posted 03/08/2016
    Samples were nuclear extracts from brown adipose and subcutaneous adipose tissue (20ug protein loaded). Primary antibody (1:1,000) was detected with HRP-anti sheep (1:10,000) then amplified with Licor streptavidin 800 (1:10,000). Near infrared fluorescence gave much better signal-to-noise than chemiluminescence.
    PRDM16/MEL1 Antibody AF6295

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Protocols

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