Human/Mouse/Rat alpha Actinin 2 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB9830R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

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FAB9830R-100UG

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Human/Mouse/Rat alpha Actinin 2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human ACTN2 in direct ELISAs.

Source

Monoclonal Rabbit IgG Clone # 2285D

Purification

Protein A or G purified

Immunogen

Synthetic peptide containing human ACTN2
Accession # P35609

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: alpha Actinin 2

Actinins are F-actin cross-linking proteins thought to anchor actin to a variety of intracellular structures. alpha-Actinin 2 (ACTN2) is a member of the spectrin superfamily with a predicted molecular weight of approximately 104 kDa. It is 894 amino acids (aa) in length and shares 99% aa identity with mouse and rat ACTN2. Two splice forms exist, resulting in 2 distinct isoforms. ACTN2 homodimerizes in an anti-parallel fashion, and can also form heterodimers with ACTN3.

Long Name

Actinin alpha 2

Entrez Gene IDs

88 (Human); 11472 (Mouse); 291245 (Rat)

Alternate Names

Actinin alpha 2; actinin, alpha 2; Actinin-a2; ACTN2; alpha Actinin 2; alpha-Actinin 2; Alpha-actinin skeletal muscle isoform 2; Alpha-Actinin Skeletal Muscle; Alpha-Actinin-2; CMD1AA; F-actin cross-linking protein