Human/Mouse/Rat Casein Kinase 1 gamma Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3186T Datasheet / COA / SDS

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AF3186T-100UG

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Human/Mouse/Rat Casein Kinase 1 gamma Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat CK1 gamma in Western blots. In Western blots, the immunogen is derived from a region of human CK1 gamma that is 97% to 98% identical to human CK1 gamma ² and CK1 gamma ³, so all three isoforms should be d

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CK1Î³
Val182-Tyr304
Accession # Q9HCP0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Casein Kinase 1 gamma

Casein Kinase 1 (CK1) represents a group of serine/threonine protein kinases that are present in all eukaryotic organisms. Human CK1 isoforms ( alpha, beta, gamma ¹, gamma ², gamma ³, δ and epsilon ) act as monomeric constitutively active enzymes that phosphorylate key regulatory proteins involved in the control of cell differentiation, proliferation, chromosome segregation and circadian rhythms. CK1 family members share a highly conserved kinase domain but differ in their variable N- and C-terminal domains. The C-termini of the gamma isoforms carry putative palmitoylation sites unique within the CK1 family that are believed to anchor the proteins in the plasma membrane.

Entrez Gene IDs

53944 (Human); 214897 (Mouse); 64086 (Rat)

Alternate Names

Casein Kinase 1 gamma; casein kinase 1, gamma 1; casein kinase I isoform gamma-1; CKI-gamma 1; CSNK1G1; EC 2.7.11; EC 2.7.11.1