Caspr2 Antibody

Detection of Caspr2 by Flow Cytometry

Expression of CASPR2 After Long-Term Exposure to CASPR2 aAbs and Structural Integrity of the VGKC Complex(A) Microfluidic chamber (MFC) with embryonic DRGs to separate somatic and axonal compartments and investigate VGKC complex organization after CASPR2 aAb incubation in separated compartments. (B) Exemplary pictures using lattice SIM2 microscopy of soma and axons from DRGs in MFCs treated with healthy control serum. Arrows mark the distance between CASPR2 (magenta) and Kv1.2 (green). (C) Quantification of distance between CASPR2 and Kv1.2 for somata (left) and axons (right) after CASPR2 aAb incubation for 2 hours. Data are shown as mean ± SEM. Somatic and axonal complexes: n = 325–1,465 and n = 1872–3,603, respectively. (D) CASPR2 and Kv1.2 density per 100 µm axon (CASPR2 n = 34–37; Kv1.2 n = 33–37). Data are shown as violin plots with individual values, median = bold line, quartiles = dotted lines. Levels of significance: ns: not significant, *p < 0.05, **p < 0.01, ****p < 0.0001. CASPR2 = Contactin-associated protein 2; DRG = dorsal root ganglia; VGKC complex = voltage-gated potassium channel complex. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40561371), licensed under a CC-BY license. Not internally tested by R&D Systems.