Human/Mouse/Rat Histone H2AX Antibody

R&D Systems | Catalog # MAB3406

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, IR Fluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 322105
View all Histone H2AX Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Histone H2AX
Gly3-Ser131
Accession # P16104

Specificity

Detects human, mouse and rat Histone H2AX in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat Histone H2AX Antibody

Detection of Human/Mouse/Rat Histone H2AX antibody by Western Blot.

Detection of Human/Mouse/Rat Histone H2AX by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Histone H2AX at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Histone H2AX antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Histone H2AX in HeLa Human Cell Line.

Histone H2AX was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AX Monoclonal Antibody (Catalog # MAB3406) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Histone H2AX by Western Blot

Detection of Histone H2AX by Western Blot

The NAMPT-dependent NAD+ salvage pathway is necessary for the recovery of NAD+ & induction of apoptosis under weak H2O2 stimulation. A The amounts of endogenous gamma H2AX & NAMPT proteins under 0.7 & 1.1 mM H2O2 stimulation determined by immunoblotting (n = 3). Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35410407), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat Histone H2AX Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line, K562 human chronic myelogenous leukemia cell line, Balb/3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line

Reviewed Applications

Read 2 reviews rated 4.5 using MAB3406 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Histone H2AX

Histone H2AX is a core histone protein that is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Alternate Names

H2AFX

Entrez Gene IDs

3014 (Human)

Gene Symbol

H2AX

UniProt

P16104

Additional Histone H2AX Products

Product Documents for Human/Mouse/Rat Histone H2AX Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Histone H2AX Antibody

For research use only

Related Research Areas

Epigenetics

Citations for Human/Mouse/Rat Histone H2AX Antibody

Customer Reviews for Human/Mouse/Rat Histone H2AX Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
0%

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Customer Images

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  • Human/Mouse/Rat Histone H2AX Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 11/04/2021
    Human/Mouse/Rat Histone H2AX Antibody MAB3406
  • Human/Mouse/Rat Histone H2AX Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 01/24/2019
    10 micrograms of mouse cortex antibody used at 1/1300 1 second exposition a: membrane after blotting with 15kDa molecular weight (*) b : membrane after blotting with 20kDa molecular weight (**)
    Human/Mouse/Rat Histone H2AX Antibody MAB3406

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Protocols

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