Human/Mouse/Rat LHX5 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF6290V Datasheet / COA / SDS

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AF6290V-100UG

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Human/Mouse/Rat LHX5 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects recombinant human LHX5 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LIM1 is observed, and less than 5% cross-reactivity with rhLHX2, and rhLHX9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human LHX5
Leu265-Trp402
Accession # Q9H2C1

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LHX5

LHX5 (LIM/homeobox protein 5) is a member of the LIM homeobox gene family. It is expressed in embryonic CNS, and appears to contribute to the formation and maintenance of multiple neuronal phenotypes. In the cerebellum, LHX5 promotes Purkinje cell development; in the spinal cord, LHX5 maintains a GABAergic phenotype in select dorsal horn interneurons. LHX5 is reported to act as a Wnt pathway antagonist through its stimulation of sFRP-1 and -5 secretion. Human LHX5 is 402 amino acids (aa) in length. It contains two LIM Zn-binding domains (aa 3-61 and 62-125), one phosphorylation site at Tyr119, and a DNA-binding homeodomain (aa 180‑239). Over aa 265-402, human LHX5 shares 97% aa identity with mouse LHX5, and 60% aa identity with its paralogue (common ancestoral) gene, LHX1.

Long Name

LIM (Lin11:Isl1:Mec3)/Homeobox Protein 5

Entrez Gene IDs

64211 (Human); 16873 (Mouse); 124451 (Rat)

Alternate Names

LHX5; LIM homeobox 5; LIM homeobox protein 5; LIM/homeobox protein Lhx5; MGC129689