Neurogranin Antibody (898502)

Neurogranin in Rat Brain.

Neurogranin was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Human/Mouse/Rat Neurogranin Monoclonal Antibody (Catalog # MAB7947) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Neurogranin by Immunohistochemistry

Expression pattern of Nrg1 in the cerebral cortex. A The densities of Nrg1-positive cells in different regions of cerebral cortex from 2-month-old Nrg1-reporting mice. P < 0.0001, n = 3 mice (6 slices) for Au and LE, n = 3 mice (9 slices) for other cortical regions, one-way-ANOVA. B The percentage of Nrg1-positive cells in different layers among total Nrg1-positive cells from multiple cortical regions. Layer factor P < 0.0001, region factor P > 0.99, n = 3 mice (9 slices) for each cortical region, two-way-ANOVA. C Diagram of mouse brain sagittal section (top) and coronal section (bottom). The line in the sagittal section diagram indicates the position of the coronal section. The rectangle indicates the brain region shown in panel D. D Fluorescent images of tdTomato in the SS1. Scale bar, 300 μm. E Fluorescent images of tdTomato, NRGN and NeuN, from the rectangle in panel D. Scale bar, 150 μm. F Fluorescent images of tdTomato, NRGN and NeuN, enlarged from the rectangle in panel E. Arrows indicate Nrg1-positive pyramidal neurons. Scale bar, 50 μm. G Fluorescent images of tdTomato and gad67-GFP in the SS1. Scale bar, 50 μm. Au: auditory cortex; RS: retrosplenial cortex; Cg1: cingulate cortex, area 1; Pir: piriform cortex; SS1: primary somatosensory cortex; LE: lateral entorhinal cortex; SS2: secondary somatosensory cortex; M1: primary motor cortex; M2: secondary motor cortex; PtA: parietal association cortex Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37147705), licensed under a CC-BY license. Not internally tested by R&D Systems.