Human/Mouse/Rat Phospho-FRS2 (Y436) Antibody

Detection of Human and Rat Phospho-FRS2 (Y436) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Bands were detected for Phospho-FRS2 (Y436) at approximately 70 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Phospho-FRS2 (Y436) in A431 Human Cell Line.

FRS2 phosphorylated at Y436 was detected in immersion fixed A431 human epithelial carcinoma cell line untreated (lower panel) or treated (upper panel) with pervanadate using Rabbit Anti-Human/Mouse/Rat Phospho-FRS2 (Y436) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5126) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Phospho-FRS2 (Y436) by Western Blot

INCB054828 inhibits FGFR-dependent signaling pathways.(A) KG1a or (B) RT-4 cells were treated with INCB054828 for 2 hours,lysed and subjected to immunoblotting for phospho- and total proteins in the FGFR signal transduction pathway including FGFR, ERK, FRS2, andSTAT5. (C) Concentration-dependent inhibition of phospho-FGFR3 byINCB054828 in RT-112 cells was determined using a proximity ligation assay with a mouse monoclonal anti-phospho-FGFR (Y653/Y654) and rabbitanti-FGFR. Original Western blot images are shown in S1 File (S1Raw images). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32315352), licensed under a CC-BY license. Not internally tested by R&D Systems.