VANGL2 (van Gogh-like protein 2; also Strabismus and Ltap) is a 65 kDa member of the Vang family of proteins. It is found in the membranes of embryonic neurons and epithelium where it associates with DVL1-3 and various frizzleds. VANGL2 mutations are associated with neural tube defects. Human VANGL2 is a 521 amino acid (aa) 4-transmembrane protein that contains an N-terminal (aa 1‑108) and C-terminal (aa 239‑521) cytoplasmic domain. There is a Ser-rich region in the N-terminus (aa 5‑20) and a PDZ-binding motif over aa 506‑521. Over aa 241‑521, human, mouse and rat show total identity in amino acid sequence.
Vang-like Protein 2/VANGL2 Antibody
R&D Systems | Catalog # AF4815
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Vang-like Protein 2/VANGL2
Gln241-Val521
Accession # Q9ULK5
Gln241-Val521
Accession # Q9ULK5
Specificity
Detects human, mouse, and rat Vang-like Protein 2/VANGL2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human VANGL1 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Vang-like Protein 2/VANGL2 Antibody
Detection of Human/Mouse/Rat Vang-like Protein 2/ VANGL2 by Western Blot.
Western blot shows lysates of mouse kidney, human and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Vang-like Protein 2/VANGL2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Vang-like Protein 2/VANGL2 in Mouse Embryo.
Vang-like Protein 2/VANGL2 was detected in immersion fixed frozen sections of mouse embryo (E15.5) using Sheep Anti-Human/Mouse/Rat Vang-like Protein 2/VANGL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4815) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Vang-like Protein 2/VANGL2 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E15.5)
Sample: Immersion fixed frozen sections of mouse embryo (E15.5)
Western Blot
1 µg/mL
Sample: Mouse kidney, human and rat brain tissue
Sample: Mouse kidney, human and rat brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Vang-like Protein 2/VANGL2
Alternate Names
LPP1, LTAP, STB1, STBM, Strabismus, Vang like Protein 2, VANGL2
Gene Symbol
VANGL2
UniProt
Additional Vang-like Protein 2/VANGL2 Products
Product Documents for Vang-like Protein 2/VANGL2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Vang-like Protein 2/VANGL2 Antibody
For research use only
Related Research Areas
Citations for Vang-like Protein 2/VANGL2 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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