Human/Mouse/Rat Vinculin Alexa Fluor® 405-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Vinculin

VCL (vinculin) is a 117 kDa actin binding cytoskeletal phosphoprotein found in adherens-type cell-cell and cell-matrix junctions. The 1066 amino acid (aa) human vinculin contains an N-terminal globular head (aa 2-835) with a talin interaction domain (aa 168-208) and three tandem repeats (aa 259-589), a pro-rich linker region (aa 826-878), and a C-terminal tail that facilitates phospholipid membrane insertion (aa 1003-1134). An 1134 aa form termed metavinculin contains 68 aa inserted after aa 915 and mainly occurs in cardiac, skeletal and smooth muscle. Human, mouse and rat vinculin share 100% aa sequence identity within the region used as an immunogen.

Product Datasheets

Related Research Areas

• Actin Stress Fibers and Focal Adhesions During EMT
• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• Extracellular Matrix Remodeling and Cell Migration During EMT
• Focal Adhesions
• Phospholipases, Small GTPases, and Other Molecules in the Akt Pathway
• Platelet Signaling