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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 692624
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Product Specifications
Immunogen
E. coli-derived recombinant human Zyxin
Lys265-Leu383
Accession # Q15942
Lys265-Leu383
Accession # Q15942
Specificity
Detects human Zyxin in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Zyxin Antibody (692624)
Detection of Human, Mouse, and Rat Zyxin by Western Blot.
Western blot shows lysates of Human cervical epithelial carcinoma cell line, PT18 mouse mast/ basophil cell line, Neuro-2A mouse neuroblastoma cell line, L6 rat myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Zyxin at approximately 82 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Zyxin in A549 Human Cell Line.
Zyxin was detected in immersion fixed A549 human lung carcinoma cell line using Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Zyxin in Rat Spinal Cord.
Zyxin was detected in perfusion fixed frozen sections of rat spinal cord using Mouse Anti-Human/Mouse/Rat Zyxin Monoclonal Antibody (Catalog # MAB6977) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to motor neurons and dorsal horn. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Zyxin by Immunocytochemistry/ Immunofluorescence
PCa cells morphology and mobility after exposure to 25, 10 kV/cm, 200 ns pulses in different frequencies with and without extracellular Ca2+. (a) The representative photographs of cells exposed to bursts of nsPEFs. Confocal laser scanning microscopy visualize the rearrangement of zyxin (green fluorescent dye), F-actin (red fluorescent dye) nucleus (blue florescent dye) structure in DU 145 cells. (b, c) The percentage of a healed wound as a function of time. Images were analyzed by ImageJ software (Version: 2.1.0/1.53C; https://imagej.net/Fiji)28. (d, e) Images of wound gradually invaded by migrating cells. Images were taken in a given time interval. The graph represents the data from the three replicates of an individual experiment. Data are mean ± SD (n = 3 replicates). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34349171), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Zyxin Antibody (692624)
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed A549 human lung carcinoma cell line
Sample: Immersion fixed A549 human lung carcinoma cell line
Immunohistochemistry
3-25 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord
Sample: Perfusion fixed frozen sections of rat spinal cord
Western Blot
0.5 µg/mL
Sample: Human cervical epithelial carcinoma cell line, PT18 mouse mast/basophil cell line, Neuro‑2A mouse neuroblastoma cell line, L6 rat myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Sample: Human cervical epithelial carcinoma cell line, PT18 mouse mast/basophil cell line, Neuro‑2A mouse neuroblastoma cell line, L6 rat myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB6977 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Zyxin
Alternate Names
Zyx, Zyxin 2
Gene Symbol
ZYX
UniProt
Additional Zyxin Products
Product Documents for Zyxin Antibody (692624)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Zyxin Antibody (692624)
For research use only
Related Research Areas
Citations for Zyxin Antibody (692624)
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: A549 Human Cell LineSpecies: HumanVerified Customer | Posted 12/22/2021
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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