Key Product Details

Species Reactivity

Human, Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1181A
View all ROR gamma t/RORC2/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

E. coli derived recombinant human ROR gamma T/RORC2/NR1F3
Met1-Gln100
Accession # P51449

Specificity

Detects recombinant human ROR gamma t/RORC2/NR1F3 by direct ELISAs and detects human and mouse ROR gamma t/RORC2/NR1F3 in flow cytometry.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

Detection of ROR?t/RORC2/NR1F3 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of ROR gamma t/RORC2/NR1F3 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC9125A) and Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (FAB3791P). Quadrant markers were set based on control antibody staining (IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
Detection of ROR?t/RORC2/NR1F3 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of ROR gamma t/RORC2/NR1F3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes either (A) unstimulated or (B) stimulated to induce Th17 cells were stained with Rabbit Anti-Human/Mouse ROR gammat/RORC2/NR1F3 APC-conjugated Monoclonal Antibody (Catalog # IC9125A) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). Quadrant markers were set based on control antibody staining (IC1051A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Detection of ROR gamma t/RORC2/NR1F3 in HepG2 cells by Flow Cytometry.

HepG2 cells were stained with Rabbit Anti-Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Monoclonal Antibody (Catalog # IC9125A, filled histogram) or isotype control antibody (Catalog # IC1051A, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Th17 cells , mouse splenocytes stimulated to induce Th17 cells and HepG2 human hepatocellular carcinoma cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ROR gamma t/RORC2/NR1F3

Retinoic acid-related Orphan Receptor gamma (ROR gamma, TOR, RORC; NR1F3) is a member of the orphan nuclear receptor family. ROR gamma is expressed in the muscle, thymus, testis, pancreas, prostate, heart, and liver. ROR gamma plays a role in thymocyte development and homeostasis. RORs bind to DNA as monomers on half-site elements with 5' A/T-rich extensions. An N-terminal isoform of ROR gamma, ROR gamma t, has been shown to be specifically expressed in the thymus.

Long Name

Retinoic Acid-Related Orphan Receptor gamma t

Alternate Names

RORC2

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449

Additional ROR gamma t/RORC2/NR1F3 Products

Product Documents for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

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Product Specific Notices for Human/Mouse ROR gamma t/RORC2/NR1F3 APC‑conjugated Antibody

For research use only

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