Semaphorin 3E Antibody

R&D Systems | Catalog # AF3239

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Primate - Chlorocebus aethiops (African Green Monkey), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Semaphorin 3E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Semaphorin 3E
Thr25-Ser775 (Arg557Ala and Arg560Ala)
Accession # O15041

Specificity

Detects human Semaphorin 3E in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Semaphorin 3A, rhSemaphorin 3C, rhSemaphorin 3F, and rhSemaphorin 3G is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Semaphorin 3E Antibody

Semaphorin 3E antibody in Mouse Brain by Immunohistochemistry (IHC-Fr).

Semaphorin 3E in Mouse Brain.

Semaphorin 3E was detected in immersion fixed frozen sections of adult mouse brain using Goat Anti-Human Semaphorin 3E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3239) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cerebellum. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Semaphorin 3E in Mouse Lung.

Immunohistochemistry (paraffin-embedded) on Native Adult rat lung tissue. Sema33 stained green, DAPI (blue). Image from a verified customer review.

Applications for Semaphorin 3E Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MDA-MB-453 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of adult mouse brain

Western Blot

0.1 µg/mL
Sample: Recombinant Human Semaphorin 3E (Catalog # 3239-S3)

Reviewed Applications

Read 1 review rated 4 using AF3239 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3E

Semaphorin 3E (Sema3E; previously SemaH) is one of six Class 3 (secreted) semaphorins which in the human share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels (2, 3). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure (4). C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (5, 6). Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins (7). Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity (7). Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (3, 8).

References

  1. Eichmann, A. et al. (2005) Genes Dev. 19:1013.
  2. Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
  3. Gu, C. et al. (2005) Science 307:265.
  4. Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
  5. Christensen, C. et al. (1998) Cancer Res. 58:1238.
  6. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  7. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  8. Gitler, A. D. et al. (2004) Developmental Cell 7:107.

Alternate Names

Sema3E, SEMAH

Entrez Gene IDs

9723 (Human); 20349 (Mouse)

Gene Symbol

SEMA3E

UniProt

O15041

Additional Semaphorin 3E Products

Product Documents for Semaphorin 3E Antibody

Certificate of Analysis

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Product Specific Notices for Semaphorin 3E Antibody

For research use only

Related Research Areas

Proteoglycans

Citations for Semaphorin 3E Antibody

Customer Reviews for Semaphorin 3E Antibody (1)

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  • Human/Mouse Semaphorin 3E Antibody
    Name: Anonymous
    Application: Immunohistochemistry (paraffin-embedded) on Native Adult rat lung tissue. Sema33 stained green, DAPI (blue).
    Sample Tested: Lung tissue
    Species: Rat
    Verified Customer | Posted 07/19/2025
    Semaphorin 3E Antibody AF3239
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.

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