Serpin F1 (serine proteinase inhibitor-clade F #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1.
Serpin F1/PEDF Antibody
R&D Systems | Catalog # AF1177
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot, Simple Western
Cited:
Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Agonist Activity
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human Serpin F1/PEDF
Asp44-Pro418
Accession # P36955
Asp44-Pro418
Accession # P36955
Specificity
Detects human Serpin F1/PEDF in direct ELISAs. Detects human and mouse Serpin F1/PEDF in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Serpin F1/PEDF Antibody
Detection of Human Serpin F1/PEDF by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Serpin F1/PEDF by Western Blot.
Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Serpin F1/PEDF in Human Kidney.
Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Serpin F1/PEDF by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 56 kDa (as indicated) using 2 µg/mL of Goat Anti-Human/Mouse Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Serpin F1/PEDF by Western Blot
PEDF did not inhibit the proliferation but inhibited the migration. (A–D) Cell numbers over time. (E–H) The decrease ratio of the cell free area by wound healing. (A) The growth curves of 143B and 143B+PEDF cells. PEDF overexpression slightly increased the cell proliferation, but the differences were not significant. (B) The cell numbers of MG63+MOC vector (MG63Neo) and MG63+PEDF in cell culture. MG63+PEDF grow slightly faster than MG63Neo. (C) The cell numbers of U2OS+mCherry vector and U2OS+PEDF in cell culture. Likely U2OS+PEDF grow faster than that of U2OS+mCherry. (D) The comparisons among Bkid, Bkid+PEDFmiR, and Bkid lamR knockout cells. The proliferations were not significantly changed by loss-of-function. (E) Wound healing abilities of 143B and 143B+PEDF cells. PEDF overexpression decreased cell motility of 143B. (F) Wound healing of MG63Neo and MG63+PEDF. MG63+PEDF delayed the healing. (G) Wound healing of U2OS+mCherry and U2OS+PEDF. Same as other cells, U2OS+PEDF significantly delayed the healing. (H) The wound healing phenotype of Bkid was cancelled by the knockdown of PEDF-lamR pathway. (I) Western blot of the effect of gene knockdown and gene knockout cell lysates. LamR knockout and PEDF knock down did not affect on the other’s expression each other. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fonc.2022.818182/full), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Serpin F1/PEDF Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin and human kidney
Sample: Immersion fixed paraffin-embedded sections of human skin and human kidney
Simple Western
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Western Blot
0.2-2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, human liver tissue, human breast tissue, and P19 mouse embryonal carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, human liver tissue, human breast tissue, and P19 mouse embryonal carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Serpin F1/PEDF
Long Name
Serpine F1/Pigment Epithelium-derived Factor
Alternate Names
EPC-1, PEDF
Gene Symbol
SERPINF1
UniProt
Additional Serpin F1/PEDF Products
Product Documents for Serpin F1/PEDF Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Serpin F1/PEDF Antibody
For research use only
Related Research Areas
Citations for Serpin F1/PEDF Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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