SEZ6L2/BSRP-A Antibody

R&D Systems | Catalog # AF4916

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Bioassay

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all SEZ6L2/BSRP-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L2/BSRP-A
Leu28-Ser837
Accession # Q4V9Z5

Specificity

Detects human and mouse SEZ6L2/BSRP-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant mouse (rm) BSRP-B and rmBSRP-C is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for SEZ6L2/BSRP-A Antibody

SEZ6L2/BSRP-A antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

SEZ6L2/BSRP-A in Mouse Embryo.

SEZ6L2/BSRP-A was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse SEZ6L2/BSRP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4916) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain (medulla oblongata), spinal cord, trigeminal ganglia, and retina. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of SEZ6L2/BSRP-A by Immunohistochemistry

Detection of SEZ6L2/BSRP-A by Immunohistochemistry

Sez6 family expression in the hippocampus. (A) Sez6, Sez6L, and Sez6L2 are expressed by principal (excitatory, pyramidal) neurons of the mouse hippocampus at much higher levels than other known complement regulators (namely Crry, C4BP, CFH, C1-INH, DAF, and MCP). Expression data was obtained from Hipposeq: a comprehensive RNA-Seq database of gene expression in hippocampal principal neurons [http://hipposeq.janelia.org (60)]. The RNA samples used in this database were isolated from mouse hippocampal principal neurons micro-dissected from the CA1, CA3, or Dentate Gyrus (DG) cell layers of the hippocampus at Postnatal Day 25-32. Differential gene expression is shown in the heatmap with the relative units of FPKM (Fragments per Kilobase of Exon per Million Reads Mapped.) (B) Brain sections from adult WT mice or Sez6 triple knockout mice (TKO) were immuno-stained for Sez6L2 (green) and DAPI and imaged in the CA1 region of the hippocampus. Scale Bar= 27 µm. High density Sez6L2 staining occurs around cell bodies in the pyramidal layer, but significant Sez6L2 is also found in the stratum radiatum and stratum oriens. (C) Higher magnification images of sections immuno-stained for Sez6L2 (green) and the postsynaptic protein, Homer1 (red), shows a subset of Sez6L2 is found near or co-localized with synapses in the stratum radiatum. Scale bar = 1.8 µm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33936031), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of SEZ6L2/BSRP-A by Flow Cytometry

Detection of SEZ6L2/BSRP-A by Flow Cytometry

Full Length Sez6L2, Sez6, and Sez6L inhibit C3b/iC3b opsonization of CHO cells by the alternative pathway. CHO cells were transfected with the indicated Myc-tagged cDNAs and then coated with a low level of antibodies and exposed to 20% C5-depleted human serum for one hour in the presence of 10 mM EGTA and 10 mM MgCl2 to block the classical pathway. Cells were then labeled with anti-C3b/iC3b and anti-Myc antibodies and analyzed by flow cytometry. (A) 5% Contour plots of C3b/iC3b versus Myc fluorescence (top layer) and C3b/iC3b fluorescence histograms (bottom layer) of the same samples normalized to mode and compared to baseline cells not exposed to serum. For Contour plots, boxed regions highlight cells designated as Myc-positive (top box) and Myc-negative (lower box) populations. For C3b/iC3b histograms, dark grey, solid line population = Myc-positive cells; Light grey, dotted line population= Myc-negative cells; White, dashed grey line population = baseline. Representative of 3+ independent experiments with technical replicates. (B) Quantification of the average median C3b/iC3b fluorescence intensity from Myc-positive and Myc-negative cells within each sample. N=3 (one experiment with three replicates; Representative of 3+ independent experiments) Statistics = t-tests. E) Average median C3b/iC3b fluorescence intensities after normalization to the Myc-negative cells from each experimental group. ANOVA (p<0.001; F(4, 10)=74.47. N=3 (3 independent experiments). For all graphs *p < 0.05; **p < 0.01 #p < 0.001 for all Myc-positive groups compared to M-CR2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33936031), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for SEZ6L2/BSRP-A Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse SEZ6L2/BSRP-A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEZ6L2/BSRP-A

SEZ6L2 (Brain-specific receptor-like protein A; also BSRP-A) is a 170 kDa member of the BSRP family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and likely regulates PKC alpha activation. Mature mouse SEZ6L2 is an 890 amino acid (aa) type I transmembrane protein. It contains an 824 aa extracellular domain (ECD) (aa 21-844) and a 43 aa cytoplasmic tail. The ECD shows two CUB domains and five short consensus repeats (SCRs). There are multiple isoforms for SEZ6L2. One shows a premature truncation after Arg403, a second shows a deletion of aa 71-130, and a third likely represents the full-length form that contains a 13 aa insertion after Val830. Over aa 28-837, mouse SEZ6L2 is 96% and 99% aa identical to human and rat SEZ6L2, respectively.

Long Name

Seizure Related 6 Homolog-Like 2/Brain-specific Receptor-like Protein-A

Alternate Names

BSRP-A, BSRPA, PSK-1

Entrez Gene IDs

26470 (Human); 233878 (Mouse); 308988 (Rat)

Gene Symbol

SEZ6L2

UniProt

Q4V9Z5 (Mouse)

Additional SEZ6L2/BSRP-A Products

Product Documents for SEZ6L2/BSRP-A Antibody

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Product Specific Notices for SEZ6L2/BSRP-A Antibody

For research use only

Citations for SEZ6L2/BSRP-A Antibody

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Protocols

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