Human/Mouse Smad9 Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Smad9

SMAD9 (SMA protein and Mothers Against Decapentaplegic 9; also SMAD8 and MAHD6) is a 55-60 kDa R-Group member of the dwarfin/SMAD family of proteins. It is expressed by BMP-responsive cells, and serves to transmit information from the BMP receptor (BMPR) to the nucleus. In particular, following BMPR activation, SMAD9 is phosphorylated by the BMPR on its C-terminus, promoting heterodimerization with SMAD4 and its translocation into the nucleus. Here, SMAD9 binds to DNA and participates in gene activation. Human SMAD9 is 467 amino acids (aa) in length. It contains a DNA-binding domain termed MH1 (aa 16-140) plus a receptor/transcription factor binding domain termed MH2 (aa 273-467). The C-terminus contains two phosphorylation sites at Ser465 and Ser467. There is one splice form that shows a deletion of aa 224-260. Over aa 158-223, human and mouse SMAD9 share 82% identy in amino acid sequence.

Product Datasheets

Related Research Areas

• Airway Inflammation and Remodeling
• Intracellular Signaling Molecules in Angiogenesis
• Mesenchymal Stem Cell Transcription Factors
• Smad Family
• Smad Transcription Factors
• TGF-beta Family
• TGF-beta Superfamily Modulators