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Human/Mouse SSEA-4 Fluorescein-conjugated Antibody

Catalog #: FAB1435F
9 Citations Datasheet / COA / SDS
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FAB1435F-100
FAB1435F-025

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Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry.
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Citations (9)
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Human/Mouse SSEA-4 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Recognizes a carbohydrate epitope of SSEA-4 (1, 2).
Source
Monoclonal Mouse IgG3 Clone # MC-813-70
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
2120Ep human embryonal carcinoma cell line
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of SSEA-4 antibody in NTera-2 human testicular embryonic carcinoma cell line antibody by Flow Cytometry. View Larger

Detection of SSEA‑4 in NTera‑2 human testicular embryonic carcinoma cell line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human/Mouse SSEA-4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1435F, filled histogram) or isotype control antibody (Catalog # IC007F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

References
  1. Shevinsky, L.H. et al. (1982) Cell 30:697.
  2. Kannagi, R. et al. (1983) EMBO J. 2:2355.
  3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.
  4. Draper, J.S. et al. (2002) J. Anat. 200:249.
Long Name
Stage-specific Embryonic Antigen-4
Alternate Names
SSEA4; SSEA-4; SSEA-4;stage-specific embryonic antigen 4

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Citations for Human/Mouse SSEA-4 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Overcoming bioprocess bottlenecks in the large-scale expansion of high-quality hiPSC aggregates in vertical-wheel stirred suspension bioreactors
    Authors: Breanna S. Borys, Tiffany Dang, Tania So, Leili Rohani, Tamas Revay, Tylor Walsh et al.
    Stem Cell Research & Therapy
  2. Optimized serial expansion of human induced pluripotent stem cells using low-density inoculation to generate clinically relevant quantities in vertical-wheel bioreactors
    Authors: Breanna S. Borys, Tania So, James Colter, Tiffany Dang, Erin L. Roberts, Tamas Revay et al.
    Stem Cells Translational Medicine
  3. Generation of the induced human pluripotent stem cell lines CSSi009-A from a patient with a GNB5 pathogenic variant, and CSSi010-A from a CRISPR/Cas9 engineered GNB5 knock-out human cell line
    Authors: N Malerba, P Benzoni, GM Squeo, R Milanesi, F Giannetti, LG Sadleir, G Poke, B Augello, AI Croce, A Barbuti, G Merla
    Stem Cell Res, 2019-08-22;40(0):101547.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Adequate taylor couette flow-mediated shear stress is useful for dissociating human iPS cell-derived cell aggregates
    Authors: K Matsuura, M Wada, K Sakaguchi, Y Matsuhashi, T Shimizu
    Regen Ther, 2019-04-25;12(0):6-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Derivation of human induced pluripotent stem cell line EURACi004-A from skin fibroblasts of a patient with Arrhythmogenic Cardiomyopathy carrying the heterozygous PKP2 mutation c.2569_3018del50
    Authors: B Ermon, CB Volpato, G Cattelan, R Silipigni, M Di Segni, C Cantaloni, M Casella, PP Pramstalle, G Pompilio, E Sommariva, V Meraviglia, A Rossini
    Stem Cell Res, 2018-09-06;32(0):78-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Generation of human induced pluripotent stem cells (EURACi001-A, EURACi002-A, EURACi003-A) from peripheral blood mononuclear cells of three patients carrying mutations in the CAV3 gene
    Authors: V Meraviglia, P Benzoni, S Landi, C Murano, M Langione, BM Motta, S Baratto, R Silipigni, M Di Segni, PP Pramstalle, D DiFrancesc, E Gazzerro, A Barbuti, A Rossini
    Stem Cell Res, 2017-12-18;27(0):25-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Xenogeneic-free defined conditions for derivation and expansion of human embryonic stem cells with mesenchymal stem cells
    Authors: Hidenori Akutsu, Masakazu Machida, Seiichi Kanzaki, Tohru Sugawara, Takashi Ohkura, Naoko Nakamura et al.
    Regenerative Therapy
  8. Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons
    Authors: Qiang Shi, Vida Hodara, Calvin R. Simerly, Gerald P. Schatten, John L. VandeBerg
    Stem Cells and Development
  9. Fibroblast growth factor-2 maintains a niche-dependent population of self-renewing highly potent non-adherent mesenchymal progenitors through FGFR2c.
    Authors: Di Maggio N, Mehrkens A, Papadimitropoulos A, Schaeren S, Heberer M, Banfi A, Martin I
    Stem Cells, 2012-07-01;30(7):1455-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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