Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 540524
View all VAMP-1/VAMP-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human VAMP-1
Met1-Lys96
Accession # P23763

Specificity

Detects human and mouse VAMP-1/VAMP-2 in Western blots. This antibody does not react with recombinant VAMP family members 5, 7, and 8.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse VAMP‑1/VAMP‑2 Antibody

Detection of Human/Mouse VAMP‑1/VAMP‑2 antibody by Western Blot.

Detection of Human/Mouse VAMP‑1/VAMP‑2 by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse VAMP-1/VAMP-2 Monoclonal Antibody (Catalog # MAB5958) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAMP-1/VAMP-2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse VAMP‑1/VAMP‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (globus pallidus)

Western Blot

0.2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, L1.2 mouse pro-B cell line, and BaF3 mouse pro-B cell line

Reviewed Applications

Read 1 review rated 5 using MAB5958 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAMP-1/VAMP-2

VAMP-1 (vesicle-associated membrane protein 1; also synaptobrevin-1/SYB1) is an 18 kDa member of the synaptobrevin family of proteins. It is expressed in neurons, neutrophils and skeletal muscle cells, and participates in vesicle fusion with the plasma membrane. Human VAMP-1 is a type IV transmembrane protein that contains an N-terminal cytoplasmic region (aa 1‑96) and a 22 aa transmembrane domain (aa 97‑118). There is one coiled-coil region between aa 33‑93. Multiple splice variants are known that show two, three, four and 81 aa substitutions for the C-terminal five amino acids. One three aa variant creates a mitochondrial targeting motif. Over aa 1‑96, human VAMP-1 is 98% aa identical to mouse VAMP-1.

Long Name

Vesicle-Associated Membrane Protein 1/2

Alternate Names

SPAX1, SYB1, VAMP1, VAMP-1, vesicle associated membrane protein 1

Entrez Gene IDs

6843 (Human); 22317 (Mouse); 25624 (Rat)

Gene Symbol

VAMP1

UniProt

P23763

Additional VAMP-1/VAMP-2 Products

Product Documents for Human/Mouse VAMP‑1/VAMP‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse VAMP‑1/VAMP‑2 Antibody

For research use only

Related Research Areas

Neuronal Lineage Markers

Customer Reviews for Human/Mouse VAMP‑1/VAMP‑2 Antibody (1)

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  • Human/Mouse VAMP-1/VAMP-2 Antibody
    Name: Steve Stowers
    Application: Immunocytochemistry
    Sample Tested: Larval ventral nerve cord and Larval neuromuscular junction
    Species: Drosophila
    Verified Customer | Posted 02/10/2018
    Drosophila third instar larval ventral nerve cord.
    Antibody recognizes Drosophila homolog of VAMP1/2 (neuronal-synaptobrevin) and gives strong staining.
    Human/Mouse VAMP‑1/VAMP‑2 Antibody MAB5958

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