Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Neurocan Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Neurocan
Glu23-Cys1321
Accession # O14594

Specificity

Detects human Neurocan in direct ELISAs. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse Neurocan is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Neurocan Antibody

Neurocan antibody in Human Brain by Immunohistochemistry (IHC-P).

Neurocan in Human Brain.

Neurocan was detected in immersion fixed paraffin-embedded sections of human brain using Sheep Anti-Human Neurocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6508) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Neurocan Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Neurocan

Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system (1). Human Neurocan contains one Ig-like V-type domain, two Link domains, two EGF-like domains, one C‑type lectin-like domain, and one Sushi domain (2). It is an approximately 300 kDa molecule of which 66 kDa is chondroitin sulfate and 60 kDa is N‑ and O‑linked glycosylation (3). Mature human Neurocan shares 66% aa sequence identity with mature mouse and rat Neurocan. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C‑terminal (Neurocan-C) fragments whose core glycoproteins are 130 kDa and 150 kDa, respectively (4, 5). The full length molecule is expressed in the developing and juvenile brain, while its cleavage products are found throughout adulthood (3, 4). Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies; Neurocan-130 is found mainly in the glial cell cytoplasm (6-8). Following brain injury, reactive astrocytes deposit increased amounts of Neurocan in the glial scar where it impedes axonal regeneration (6, 9). Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules including NCAM-L1, NCAM-1, Syndecan-3, Glypican-1, Tenascin, Contactin-2/TAG1, and HAPLN1 (1, 6, 10-15).

References

  1. Rauch, U. et al. (2001) Cell. Mol. Life Sci. 58:1842.
  2. Prange, C.K. et al. (1998) Gene 221:199.
  3. Rauch, U. et al. (1991) J. Biol. Chem. 266:14785.
  4. Matsui, F. et al. (1994) Neurochem. Int. 25:425.
  5. Rauch, U. et al. (1992) J. Biol. Chem. 267:19536.
  6. Asher, R.A. et al. (2000) J. Neurosci. 20:2427.
  7. Matsui, F. et al. (1998) Brain Res. 790:45.
  8. Abaskharoun, M. et al. (2010) Brain Res. 1327:6.
  9. Shen, L.H. et al. (2008) Glia 56:1747.
  10. Friedlander, D.R. et al. (1994) J. Cell Biol. 125:669.
  11. Sango, K. et al. (2003) Exp. Neurol. 182:1.
  12. Akita, K. et al. (2004) Biochem. J. 383:129.
  13. Grumet, M. et al. (1994) J. Biol. Chem. 269:12142.
  14. Milev, P. et al. (1996) J. Biol. Chem. 271:15716.
  15. Rauch, U. et al. (2004) Matrix Biol. 22:629.

Alternate Names

CSPG3, NCAN, NEUR

Entrez Gene IDs

1463 (Human); 13004 (Mouse); 58982 (Rat)

Gene Symbol

NCAN

UniProt

O14594

Additional Neurocan Products

Product Documents for Human Neurocan Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Neurocan Antibody

For research use only

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Protocols

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