Human NF-H Antibody

R&D Systems | Catalog # AF3108

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all NF-H Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NF-H
Met1-Ala380
Accession # P12036

Specificity

Detects human NF-H in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) NF-M and rhNF-L is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human NF-H Antibody

NF-H antibody in Human Brain by Immunohistochemistry (IHC-P).

NF-H in Human Brain.

NF-H was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using 15 µg/mL Goat Anti-Human NF-H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Human NF‑H by Simple WesternTM.

Simple Western shows lysates of human cerebellum, loaded at 0.2 mg/ml. A specific band was detected for NF‑H at approximately 373 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human NF‑H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3108). This experiment was conducted under reducing conditions and using the 66-440kDa separation system.

Applications for Human NF-H Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Simple Western

2.5 µg/mL
Sample: Human cerebellum

Western Blot

0.1 µg/mL
Sample: Human NF-H

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NF-H

Neurofilament (NF)-H is a 200 kDa neurofilament protein and is one of three NF subunits. In the protein region used for immunization, human NF-H shares approximately 92% sequence identity with the mouse or rat protein.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human)

Gene Symbol

NEFH

UniProt

P12036

Additional NF-H Products

Product Documents for Human NF-H Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NF-H Antibody

For research use only

Citations for Human NF-H Antibody

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Protocols

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