Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 915911
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human NKCC1/SLC12A2
Met1-Ser1212
Accession # P55011
Met1-Ser1212
Accession # P55011
Specificity
Stains NKCC1/SLC12A2 transfectants but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human NKCC1/SLC12A2 Antibody
Detection of NKCC1/SLC12A2 in HEK293 Human Cell Line Transfected with Human NKCC1/SLC12A2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP was stained with either (A) Mouse Anti-Human NKCC1/SLC12A2 Monoclonal Antibody (Catalog # MAB8528) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Applications for Human NKCC1/SLC12A2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human NKCC1/SLC12A2 and eGFP
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: NKCC1/SLC12A2
Long Name
Sodium/Potassium/Chloride Transporter NKCC1
Alternate Names
BSC2, SLC12A2
Gene Symbol
SLC12A2
UniProt
Additional NKCC1/SLC12A2 Products
Product Documents for Human NKCC1/SLC12A2 Antibody
Product Specific Notices for Human NKCC1/SLC12A2 Antibody
For research use only
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