Osteoprotegerin is a member of the TNF receptor superfamily expressed by many cell types. It exists in soluble monomeric and dimeric forms as a decoy receptor for its ligands, TRANCE and TRAIL. Osteoprotegerin functions as an antagonist to RANKL-induced osteoclastogenesis and bone resorption.
Human Osteoprotegerin/TNFRSF11B Antibody (69127)
R&D Systems | Catalog # MAB8051
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Antibody Array Development, ELISA Capture, ELISA Development, ELISA Development (Capture)
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 69127
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Osteoprotegerin/TNFRSF11B
Glu22-Leu401
Accession # O00300
Glu22-Leu401
Accession # O00300
Specificity
Detects human Osteoprotegerin/TNFRSF11B in ELISAs. It has been reported (1), and R&D Systems testing has confirmed, that this antibody shows strong cross-reactivity with an unrelated protein, human Carbonic Anhydrase II. In Western blots, this antibody does not cross‑react with recombinant human (rh) 4‑1BB, rhCD27, rhCD30, recombinant mouse (rm) CD30, rmCD40, rhDR3, rhDR6, rhFas, rmFas, rhGITR, rmGITR, rhHVEM, rhLT beta R, rhNGF R, rmOPG, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Osteoprotegerin/TNFRSF11B Antibody (69127)
Human Osteoprotegerin / TNFRSF11B ELISA Standard Curve
Recombinant Human Osteoprotegerin/TNFRSF11B (Catalog # 185-OS) was serially diluted and captured by Mouse Anti-Human Osteoprotegerin/TNFRSF11B Monoclonal Antibody (Catalog # MAB8051) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Osteoprotegerin/TNFRSF11B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF805) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human Osteoprotegerin/TNFRSF11B Antibody (69127)
Human Osteoprotegerin/TNFRSF11B Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB8051 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Osteoprotegerin/TNFRSF11B
References
- Waterman, E.A. et al. (2007) Intl. J. Cancer 121:1958.
Alternate Names
OCIF, TNFRSF11B
Gene Symbol
TNFRSF11B
UniProt
Additional Osteoprotegerin/TNFRSF11B Products
Product Documents for Human Osteoprotegerin/TNFRSF11B Antibody (69127)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Osteoprotegerin/TNFRSF11B Antibody (69127)
For research use only
Related Research Areas
Citations for Human Osteoprotegerin/TNFRSF11B Antibody (69127)
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Customer Reviews for Human Osteoprotegerin/TNFRSF11B Antibody (69127) (1)
5 out of 5
1 Customer Rating
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 07/19/2016
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Associated Pathways
