Human PD-L1 Antibody

Catalog # Availability Size / Price Qty
MAB1561
MAB1561-SP
Human PD-L1 Antibody in Data
3 Images
Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (5)

Human PD-L1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PD-L1/B7-H1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, -2, -H2, -H3, -H3b,
-H4, rhPD-L2, recombinant mouse B7-H1, recombinant rat (rr) B7-1, or rrB7-2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 130021
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human PD-L1/B7-H1
Phe19-Thr239
Accession # Q9NZQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of PD-L1/B7-H1 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry. View Larger

Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry PD-L1/B7-H1 in Human Colon Cancer. View Larger

PD-L1/B7-H1 in Human Colon Cancer. PD-L1/B7-H1 was detected in formalin fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was observed in the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

References
  1. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  2. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
  3. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.
Long Name
Programmed Death Ligand 1
Entrez Gene IDs
29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names
Avelumab; B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1

Product Datasheets

Citations for Human PD-L1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
Filter your results:

Filter by:

  1. Accumulation of circulating CCR7+ natural killer cells marks melanoma evolution and reveals a CCL19-dependent metastatic pathway
    Authors: CM Cristiani, A Turdo, V Ventura, T Apuzzo, M Capone, G Madonna, D Mallardo, C Garofalo, ED Giovannone, AM Grimaldi, R Tallerico, E Marcenaro, S Pesce, G Del Zotto, V Agosti, FS Costanzo, E Gulletta, A Rizzo, A Moretta, K Kärre, PA Ascierto, M Todaro, E Carbone
    Cancer Immunol Res, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
    Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
    PLoS ONE, 2019;14(5):e0215990.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Detection
  3. Soluble PD-L1 as a biomarker in malignant melanoma and checkpoint blockade
    Authors: J Zhou, KM Mahoney, A Giobbie-Hu, F Zhao, S Lee, X Liao, S Rodig, J Li, X Wu, LH Butterfiel, M Piesche, MP Manos, LM Eastman, G Dranoff, GJ Freeman, FS Hodi
    Cancer Immunol Res, 2017;0(0):.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Capture)
  4. Hilar fat infiltration: A new prognostic factor in metastatic clear cell renal cell carcinoma with first-line sunitinib treatment
    Authors: SF Kammerer-J, A Brunot, K Bensalah, B Campillo-G, M Lefort, S Bayat, A Ravaud, F Dupuis, M Yacoub, G Verhoest, B Peyronnet, R Mathieu, A Lespagnol, J Mosser, J Edeline, B Laguerre, JC Bernhard, N Rioux-Lecl
    Urol. Oncol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  5. Overexpression of B7-H1 correlates with malignant cell proliferation in pancreatic cancer.
    Authors: Song, Xiao, Liu, Junwei, Lu, Yi, Jin, Hongchua, Huang, Dongshen
    Oncol Rep, 2014;31(3):1191-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.
    Authors: Rojas D, Krishnan R,
    Immunol. Lett., 2010;132(1):31-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Frequent expression of PD-L1 on circulating breast cancer cells.
    Authors: Mazel M, Jacot W, Pantel K, Bartkowiak K, Topart D, Cayrefourcq L, Rossille D, Maudelonde T, Fest T, Alix-Panabieres C
    Mol Oncol, 0;9(9):1773-82.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Independent association of PD-L1 expression with noninactivated VHL clear cell renal cell carcinoma-A finding with therapeutic potential.
    Authors: Kammerer-Jacquet S, Crouzet L, Brunot A, Dagher J, Pladys A, Edeline J, Laguerre B, Peyronnet B, Mathieu R, Verhoest G, Patard J, Lespagnol A, Mosser J, Denis M, Messai Y, Gad-Lapiteau S, Chouaib S, Belaud-Rotureau M, Bensalah K, Rioux-Leclercq N
    Int J Cancer, 0;140(1):142-148.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human PD-L1 Antibody

Average Rating: 4.8 (Based on 5 Reviews)

5 Star
80%
4 Star
20%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human PD-L1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human PD-L1 Antibody
By Anonymous on 11/04/2020
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human

Human PD-L1/B7-H1 Antibody
By Anonymous on 12/16/2017
Application: SPR ASSAY Sample Tested: MCF7 Species: Human

Human PD-L1/B7-H1 Antibody
By Anonymous on 12/11/2017
Application: IHC Sample Tested: Colon cancer tissue Species: Human

Human PD-L1/B7-H1 Antibody
By Anonymous on 12/04/2017
Application: SPR Sample Tested: Human recombinant test antibody Species: Mouse

Human PD-L1/B7-H1 Antibody
By Anonymous on 11/20/2017
Application: Flow Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human