Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PDCD4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDCD4
Lys212-Pro357
Accession # Q53EL6

Specificity

Detects human PDCD4 in direct ELISAs.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PDCD4 Antibody

PDCD4 antibody in Human Stomach by Immunohistochemistry (IHC-P).

PDCD4 in Human Stomach.

PDCD4 was detected in immersion fixed paraffin-embedded sections of human stomach using Sheep Anti-Human PDCD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7019) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
PDCD4 antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

PDCD4 in MCF-7 Human Cell Line.

PDCD4 was detected in immersion fixed MCF-7 human breast cancer cell line using Sheep Anti-Human PDCD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7019) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PDCD4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Reviewed Applications

Read 2 reviews rated 4.5 using AF7019 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDCD4

PDCD4 (Programmed cell death protein 4; also H731) is a 54-64 kDa member of the PDCD4 family of molecules. It is widely expressed, being found in mammary epithelium, CD34+ bone marrow progenitor cells, fibroblasts and keratinocytes. PDCD4 is both cytoplasmic and nuclear. In the cytoplasm, it blocks protein translation by binding to eIF4A, an act that dissociates eIF4G and mRNA from eIF4A. In the nucleus, it seems to block transcription of select genes, one of which is MAP4K1, a key enzyme in the AP-1-mediated transcription pathway. Human PDCD4 is 469 amino acids (aa) in length. It contains an NLS (aa 58-64), an MI domain (aa 163-284), another NLS (aa 241-250) and a second MI domain (aa 326-449). There are at least seven utilized Ser/Thr phosphorylation sites and one Tyr phosphorylation site. There are two potential splice forms, one that contains a deletion of aa 15-28, and a second that may show a three aa substitution for aa 2-15. Over aa 212-357, human PDCD4 shares 98% aa identity with mouse PDCD4.

Long Name

Programmed Cell Death 4

Alternate Names

H731, Protein 197/15a

Entrez Gene IDs

27250 (Human); 18569 (Mouse); 64031 (Rat)

Gene Symbol

PDCD4

UniProt

Q53EL6 (Human)

Additional PDCD4 Products

Product Documents for Human PDCD4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PDCD4 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Translation Regulation

Customer Reviews for Human PDCD4 Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
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2 Stars
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1 Stars
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  • Human PDCD4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Prostate array
    Species: Human
    Verified Customer | Posted 01/26/2018
  • Human PDCD4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Tonsil tissue
    Species: Human
    Verified Customer | Posted 12/16/2017

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Protocols

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