Human Perilipin-2 Antibody (788616)

R&D Systems | Catalog # MAB76341

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 788616
View all Perilipin-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Perilipin-2
Met1-His437
Accession # Q99541

Specificity

Detects human Perilipin-2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Perilipin-2 Antibody (788616)

Detection of Human Perilipin-2 antibody by Western Blot.

Detection of Human Perilipin-2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Huh-7 human hepatoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Perilipin-2 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Perilipin-2 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Perilipin-2 in HepG2 Human Cell Line.

Perilipin-2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Perilipin-2 antibody in Human Liver by Immunohistochemistry (IHC-P).

Perilipin-2 in Human Liver.

Perilipin-2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Perilipin-2 Monoclonal Antibody (Catalog # MAB76341) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Perilipin-2 by Immunohistochemistry

Detection of Perilipin-2 by Immunohistochemistry

Interstitial changes in intimomedial tears.Aortas were analyzed by histology and immunofluorescence confocal microscopy. (A) Erythrocyte marker glycophorin-A (green color) is not detected in distant media or tear neointima, although red blood cells are seen in adventitial microvessels (inset). (B) Absent Perls Prussian blue/diaminobenzidine (DAB) staining for iron (brown) in tear neointima, but strong staining in positive control of chronic aortic dissection (inset). (C) Medial extravasation of red blood cells (red) from nearby dissection is restricted (arrow) by fibrotic neointima of intimomedial tear. (D) Albumin (green) is not detected in tear neointima, but is found in positive control of acute aortic dissection (inset). (E) Fibrin (green) is detected in tear neointima, but not distant media, with organization into dense structures at tear edges. (F) Oil red O staining for neutral lipid (red) is absent in distant media and tear neointima, weak in neointima overlying distant media, and strong in aortic atheroma without intimomedial tear. (G) Perilipin-1 (green) and (H) perilipin-2 (green) are not detected in SMCs of distant media or tear neointima but are present in adventitial adipocytes and plaque foam cells (insets), respectively. Black scale bars: 250 μm; white scale bars: 25 μm. Image collected and cropped by CiteAb from the following open publication (https://insight.jci.org/articles/view/172437), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Perilipin-2 Antibody (788616)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Huh‑7 human hepatoma cell line

Reviewed Applications

Read 1 review rated 5 using MAB76341 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Perilipin-2

Perilipin-2 (PLIN2), also known as Adipophilin (ADFP) and Adipose Differentiation-Related Protein (ADRP), is a widely expressed 52 kDa acylated protein that associates with membranes and cytoplasmic lipid droplets. It is upregulated during adipocyte differentiation and is a major component of milk fat globules. Perilipin-2 promotes the cellular uptake and accumulation of long chain fatty acids and regulates the intracellular distribution of phospholipids. It promotes hepatic insulin resistance in response to a high fat diet. Human Perilipin-2 shares 82% and 79% aa sequence identity with mouse and rat Perilipin-2, respectively.

Alternate Names

ADFP, Adipophilin, Perilipin2, PLIN2

Entrez Gene IDs

123 (Human); 11520 (Mouse); 298199 (Rat)

Gene Symbol

PLIN2

UniProt

Q99541 (Human)

Additional Perilipin-2 Products

Product Documents for Human Perilipin-2 Antibody (788616)

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Product Specific Notices for Human Perilipin-2 Antibody (788616)

For research use only

Citations for Human Perilipin-2 Antibody (788616)

Customer Reviews for Human Perilipin-2 Antibody (788616) (1)

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  • Human Perilipin-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adipocytes
    Species: Human
    Verified Customer | Posted 10/28/2021
    Human Perilipin-2 Antibody (788616) MAB76341

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