Human Phospho-Akt1 (T308) Alexa Fluor® 700-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Akt1

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain. Akt1 is the most widely expressed family member and is frequently activated in a number of carcinomas, including breast, prostate, lung, pancreatic, liver, ovarian, and colorectal cancer. Akt1 is activated in a multistep process that involves the sequential phosphorylation of Thr450 by JNK kinases, Thr308 by PDK1, and Ser473 by PDK2 or mTORC2. Activated Akt1 phosphorylates a wide variety of cytosolic, nuclear, and mitochondrial substrates. Human Akt1 shares 98% aa sequence identity with mouse and rat Akt1. MAB7419 also detects mouse Phospho-Akt1 (T308) in Western Blot.

Product Datasheets

Related Research Areas

• Apoptosis Intracellular Kinases
• Chemokine Receptor Intracellular Signaling
• Common gamma Chain Receptor Family
• Diabetic Peripheral Neuropathy
• GDNF Family
• Growth Hormone/IGF-I Axis
• IL-10 Family
• Interferons
• Intracellular Kinases
• Intracellular Kinases in the Akt Pathway
• Intracellular Signaling Molecules in Angiogenesis
• Kinases and Phosphatases in Endothelial Cells
• Neurofibrillary Tangle Formation
• Neurotrophin/Trk Family
• Notch Pathway
• PDGF Family
• Platelet Signaling
• Search Kinases and Phosphatases
• TNF Superfamily Regulators
• VEGF Family